家蚕表皮基因BmGCP2的克隆及表达谱分析

Molecular Cloning and Expression Analysis of BmGCP2 Gene in Epidermis of Bombyx mori

从家蚕(Bombyxmori)表皮中克隆了一个高丰度表达的基因,根据其编码的氨基酸序列特征,发现是一个富含甘氨酸的表皮蛋白,将该基因命名为BmGCP2(GenBank登录号:EU980611)。进一步对BmGCP2基因进行了组织表达谱和发育时期表达谱分析,发现该基因在家蚕表皮中特异高表达,并且这种高表达一直从家蚕的胚胎发育后期持续到蛾期,表明BmGCP2基因编码的表皮蛋白是家蚕外骨骼的重要组成成分之一。

In present study, an abundantly expressed gene was cloned from the epidermis of Bombyx mori. According to the sequence characteristic of its encoded protein, it was a glycine-rich epidermal protein and was thus termed BmGCP2 （GenBank accession number EU980611 ）. We further investigated its expression profiles in different tissues and at different developmental stages. The results indicated that the expression of BmGCP2 was maintained at high levels in epidermis of Bombyx mori. Moreover, this high level expression was detected from the late embryogenesis stage to adult stage, suggesting that the epidermal protein encoded by BmGCP2 is one of the crucial components of the exoskeleton in Bombyx mori.