预测鼠疫耶尔森氏菌F1抗原的CTL表位

CTL epitope prediction on F1 antigen of Yersinia pestis

目的预测鼠疫菌F1抗原的CTL表位,为鼠疫菌表位疫苗研究奠定基础,并为绘制鼠疫菌抗原表位图谱提供一种可行的研究手段。方法采用nHLAPred网络预测法对F1抗原的CTL表位进行预测。结果预测得到了鼠疫菌F1抗原的特异性CTL优势表位:120～128 SPKVNGENL和153～161 KLAAGKYTD。结论利用nHLAPred法,并结合其他T细胞表位预测方法,可得到抗原的CTL表位,准确率较高,可作为表位图谱绘制的有效工具。

Objective To predict the CTL epitopes of F1 antigen in order to set up a basis for the research on epitope vaccine of plague and at the same time, offer a feasible way for epitope mapping of Yersinia pestis antigens. Methods The method of nHLAPred was adopted to predict the CTL epitopes. Results F1 CTL epitopes were 120-128 SPKVNGENL and 153-161 KLAAGKYTD. Conclusions The method of nHLAPred can be used for CTL epitope prediction because of its accuracy. It is a good method to make epitope mapping.