摘要
采用反相高效液相色谱法(RP-HPLC)对乳腺增生丸中柴胡皂苷a、d含量进行测定。色谱柱为AgilentE-clipse XDB-C18(4.6mm×250mm,5μm),以乙腈∶水(33∶67)为流动相,检测波长为210nm。结果表明,柴胡皂苷a在6.92～23.07μg.mL-1(R=0.9996)范围内、柴胡皂苷d在5.95～19.84μg·mL-1(R=0.9997)范围内,浓度与峰面积呈良好线性关系,方法平均回收率分别为99.28%(RSD=0.79%)、98.32%(RSD=1.11%)。该方法对乳腺增生丸中柴胡皂苷a、d含量的测定简便易行,结果准确可靠,适用于对乳腺增生丸进行质量控制。
The content of saikosaponin a and saikosaponin d in Ruxianzengsheng Pill have been determined by RP-HPLC method. The chromatographic column is Agilent Eclipse XDB-C18 （4.6 mmX 250 ram, 5 μm ）. Mobile phase is acetonitrile and water solution with ratio of 33 ： 67. The detection wavelength is 210 nm. There are good linear relationships between the concentration of 6.92-23.07 μg · mL^-1 and peak area of saikosaponin a （R=0. 9996） and the concentration of 5.95-19.84μg · mL^-land peak area of saikosaponin d（R= 0. 9997）. The recovery of saikosaponin a is 99. 28% and relative standard deviation is 0.79%. The recovery of saikosaponin d is 98.32% and relative standard deviation is 1.11%. The present method for determining con- tent of saikosaponin a and saikosaponin d in Ruxianzengsheng Pill is simple, feasible with accurate and reliable results, which is suitable for the quality control of this preparation.
出处
《化学与生物工程》
CAS
2009年第9期88-90,共3页
Chemistry & Bioengineering
