子宫颈癌与HPV感染关系的临床研究

Clinical study of relationship between cervical cancer and HPV infection

目的分析子宫颈癌组织HPV检测的结果及相关临床资料,探讨HPV与子宫颈癌的临床生物学行为之间关系。方法收集200例有完整临床病历资料的子宫颈癌患者病灶组织,采用HPV分型基因芯片检测系统,进行HPV阳性率及其亚型的检测并对相关资料加以分析。结果HPV检出率94.0%,其中HPV16阳性率65.6%;HPV18阳性率15.5%。HPV阳性率与子宫颈癌临床分期、病理分级无相关性(P=0.25、P=0.75);HPV阳性率与肿瘤的盆腔淋巴结转移、肿瘤的治疗后复发亦无相关性(P=0.29、P=0.47);鳞状细胞癌的HPV检出率明显高于腺癌(P<0.005)。结论子宫颈癌组织中的HPV阳性率很高,主要为16、18型。HPV感染与鳞状上皮恶变的关系更密切;HPV与宫颈癌的临床期别、病理分级、盆腔淋巴结转移、肿瘤的复发无明显相关性。

Objective To explore the relationship between HPV infection and clinical biological behavior of cervical cancer through the analysis of HPV examination result and clinical data in cervical cancer. Methods The tissue samples of 200 cervical cancer patients who had complete clinical data were collected. HPV genotyping chip system was used to study the HPV positive rate and subtypes. Results HPV positive rate was 94. 0%, among which HPV 16 was 65. 0% and HPV 18 was 15.5%. HPV positive rate was not related to clinical stage and pathological grade（P =0. 25 ,P =0. 75）. HPV positive rate was also not related to the metastasis to lymph nodes and the recurrence of tumor after treatment（ P = 0. 29 ,P = 0. 47 ）. HPV posifive rate was higher in squamous cell carcinoma than in adenocarcinoma （ P 〈 0. 005 ）. Conclusions HPV positive rate was high in cervical cancer and HPV 16 and 18 were the main types. HPV infection was more closely related with the malignant change of squamous cells, and was not related to clinical stage, pathological grade, metastasis to lymph nodes and recurrence of cervical cancer.