caspase-10在甲状腺分化癌中的表达及临床意义

The expression of caspase-10 in differentiated thyroid carcinoma and association with its development and metastasis

目的探讨caspase-10表达在甲状腺分化癌（DTC）发生、发展及预后中的作用。方法37例DTC来自于2006年1月至2007年12月行甲状腺切除术患者。取DTC患者病理标本，链霉菌抗生物素蛋白-过氧化物酶连结法（S—P）染色，光镜下检测DTC患者caspase-10表达，分析caspase-10在DTC中的表达与临床病理特征之间的关系。取同期46例结节性甲状腺肿组织标本作为对照。结果结节性甲状腺肿细胞质或细胞膜。可见到较多的棕色或黄色caspase-10表达颗粒，但在DTC中棕色或黄色表达颗粒不明显。caspase-10表达阳性率在DTC患者中为29．73％（11／37），在结节性甲状腺肿患者中为71．74％（33／46），二者比较差异有统计学意义（χ^2=14．528，P〈0．01）；在DTC有淋巴结转移患者中，caspase-10阳性表达率（11．11％，2／18）明显低于无淋巴结转移患者（47．37％，9／19），二者比较差异有统计学意义（χ^2=4．210，P〈0．01）；caspase-10表达与性别、年龄、国际肿瘤（TNM）分期以及病理分型无关（P〉0．05）。结论caspase-10表达下调在甲状腺分化癌的发生和侵袭中发挥重要作用。

Objective To investigate the expression of caspase-10 in differentiated thyroid carcinoma and association with its development and metastasis. Methods Thyroid samples from 37 patients in a period from January 2006 to December 2007, with differentiated thyroid carcinoma were retrospectively analyzed for caspase-10 by immunohistochemistry（streptavidin-perosidase,S-P）, compared to control group of 46 cases with nodular goiter. The relationship between the expression of caspase-10 and the clinical pathologic characteristics of thyroid carcinoma were also explored simultaneously. Results caspase-10 were observed as brown or yellow particles located in the cytoplasm or cell membrane of nodular goiter but there were no significant evidence for its positive expression in thyroid carcinoma, caspase-10 expression was markedly down-regulated in differentiated thyroid carcinoma（29.73%, 11/37） compared with benign nodules （71.74%, 33/46, χ^2 = 14.528, P 〈 0.01）. The positive expression in 18 cases with lymph node metastasis（11.11%,2/18）was significantly lower than those in 19 patients without lymph node metastasis（47.37%,9/19; χ^2= 4.210, P 〈 0.01）. There was no significant correlation （P 〉 0.05） between the expression of caspase-10 and the clinical pathologic characteristics including male, age, TNM stage and pathologic type. Conclusion Down-regulation of caspase-10 may play a critical role in carcinogenesis and development of differentiated thyroid carcinoma.