阿苯达唑联合黄芪对华支睾吸虫感染大鼠肝功能异常的疗效观察

Efficacy of the abnormal liver function in rats infected with Clonorchis sinensis after treatment of albendazole combined with milkvetch root

目的观察阿苯达唑联合黄芪对华支睾吸虫感染大鼠肝功能异常的疗效。方法健康成年Wistar大鼠32只，按体质量随机分为对照组、未治疗组、阿苯达唑组和阿苯达唑＋黄芪组,每组8只。未治疗组、阿苯达唑组和阿苯达唑＋黄芪组大鼠给华支睾吸虫囊蚴50个／只灌胃．对照组大鼠给生理盐水灌胃：阿苯达唑组大鼠在感染后10周每日每只给阿苯达唑50mg／kg灌胃，连续5d：阿苯达唑＋黄芪组大鼠在给阿苯达唑的同时腹腔注射黄芪注射液800mg／kg，连续30d。于感染后14周采集血样，检测血清丙氨酸转氨酶（ALT）、总胆红素（TBIL）、透明质酸酶（HA）、层粘连蛋白（LN）和Ⅲ型前胶原（PCⅢP）水平。结果大鼠血清ALT和LN水平组间比较，差异有统计学意义（F值分别为31．40、11．82，P〈0．01）。血清ALT和LN水平，未治疗组[（85．50±9．65）U／L、（64．20±4．18）μg／L]、阿苯达唑组[（65．29±7．78）U／L、（58．23±2．55）μg／L]较对照组[（47．88±4．88）U／L、（51．20±4．12）μg／L]明显升高（P〈0．05）；阿苯达唑组和阿苯达唑＋黄芪组[（50．25±9．29）U／L、（53．68±5．63）μg／L]较未治疗组明显下降（P〈0．05），阿苯达唑＋黄芪组下降较阿苯达唑组更明显（P〈0．05）。大鼠血清TBIL、PⅢNP和HA组间比较差异有统计学意义（χ^2值分别是15．309、21．418、19．759，P〈0．01）。血清TBIL、PⅢNP、HA水平，未治疗组（2．400μmol／L、46．220μg／L、310．885μg／L）、阿苯达唑组（1．200μmol／L、36．540μg／L、178．010μg／L）较对照组（0．700μmol／L、26．085μg／L、81．935μg／L）明显升高（P〈0．05）。大鼠血清TBIL水平，阿苯达唑＋黄芪组（0．750μmol／L）较未治疗组明显降低（P〈0．05）；大鼠血清PⅢNP和HA水平，阿苯达唑组和阿苯达唑＋黄芪组（30．470μg／L、100．240μg／L）较未治疗组明显降低（P〈0．05），与阿苯达唑组比较，阿苯达唑＋黄芪组大鼠血清TBIL、PⅢNP和HA水平下降更明显（P〈0．05）。结论大鼠感染华支睾吸虫后肝功能异常，经阿苯达唑联合黄芪治疗后肝功能得到改善，肝纤维化减轻，阿苯达唑联合黄芪治疗效果好于单独应用阿苯达唑治疗。

Objective To observe the levels of alanine aminotransferase（ALT）, total bilirubin（TBIL）, hyaluronic acid （ HA ）, procollagen type m aminoterminal peptide （P In NP ） and laminin （LN） in the sera of rats infected with Clonorchis sinensis （C. sinensis ） after treatment of albendazole combined with milkvetch root. Methods Thirty-two healthy adult Wistar rats were randomly divided into four groups with 8 in each based on body mass： control group, non-treatment group, Albendazole group （ALB group） and albendazole combined with milkvetch root group（ALB ＋ MR group）. The rats in non-treatmen, ALB and ALB ＋ MR groups were infected orally with metacercariae of C. sinensis 50 per rat. The rats in control group were mock-infected with saline. The rats in ALB group were treated to each rat with 50 mg/kg albendazole for 5 days, and ALB ＋ MR groups were given to same treat with albendazole, meanwhile each rat injected with 800 mg/kg milkvetch root intraperitoneally for 30 days. All rats were killed after infestation 14 weeks and their sera samples were collected to detect ALT, TBIL, HA, P HI NP, LN. Results There were statistically significant differences in the levels of ALT and LN in the sera of rats between groups （F = 31.40,11.82, P 〈 0.01 ）. Compared with control [ （47.88 ± 4.88）U/L, （51.20± 4.12 ） μg/L ], the levels of ALT and LN in rats in non-treatment group[ （85.50 ± 9.65）U/L, （64.20 ± 4.18）μg/L] and ALB group[ （65.29± 7.78）U/L, （58.23 ±2.55 ）μg/L ] were significantly increased （P 〈 0.05 ）. Compared with non-treatment group, the levels of ALT and LN in rats in ALB group and ALB ＋ MR groups[ （50.25± 9.29）U/L, （53.68 ± 5.63）μg/L] were significantly decreased（P 〈 0.05）, and they decreased more obviously in ALB ＋ MR group （P 〈 0.05）. There were statistically signifieant differences in the levels of TBIL, P Ⅲ NP and HA in the sera of rats between groups （χ^2= 15.309,21.418,19.759, P 〈 0.01 ）. Compared with control[ （0.700 ± 0.350）μmol/L, （26.085 ±4.075）Hg/L, （81.935± 42.550）μg/L], the levels of TBIL, PⅢNP and HA in rats in non-treatment group（2.400 μmol/L, 46.220 μg/L, 310.885 μ/L） and ALT group （ 1.200 μmol/L,36.540 μg/L, 178.010 μg/L） were significantly inereased（P 〈 0.05）. Compared with non-treatment group, the level of TBIL in ALT ＋ MR group（0.750 μmol/L）, the levels of PⅢNP and HA in ALT and ALT ＋ MR group（30.470,100.240 μg/L） were significantly deereased（P 〈 0.05）. The levels ofTBIL, PmNP and HA decreased more obviously in ALB ＋ MR group（P 〈 0.05）. Conclusions The liver function in rats infected with C. sinensis is abnormal. The liver function and fibrosis are improved after treatment with albendazole or albendazole combined with milkvetch root. The treatment of albendazole combined with milkvetch root is more effective.