经动脉灌注新辅助化疗对局部进展期乳腺癌激素受体表达水平的上调作用

Transarterial infusion neoadjuvant chemotherapy up-regulates expressions of hormone receptors in local advanced breast cancer tissues

目的探讨经动脉灌注化疗对乳腺癌雌激素(estrogen receptor,ER)和孕激素受体(progesterone receptor,PR)表达的影响。方法应用逆转录聚合酶链式反应(RT-PCR)和免疫组化方法检测81例乳腺癌患者经动脉灌注化疗前后的雌孕激素受体表达情况。采用Western blot检测经动脉灌注化疗前后ER和PR的变化。结果RT-PCR结果表明,81例患者中43例(53.1%)ERmRNA水平升高,33例(40.7%)PRmRNA水平升高,25例(30.9%)ER和PRmRNA水平同时升高。免疫组织化学结果提示,35例(43.2%)ER表达水平升高,其中14例(17.3%)由阴性转为阳性;21例(26.0%)PR表达水平升高,其中10例(12.2%)由阴性转为阳性;16例(19.8%)ER和PR表达同时升高。Wester nblot显示,经动脉灌注化疗后,81例患者中38例(46.9%)ER蛋白水平升高,30例(37.0%)PR蛋白水平升高,21例(25.9%)ER和PR蛋白水平同时升高。结论经动脉灌注化疗可有效上调ER、PRmRNA和蛋白水平的表达,有利于后续化疗药物对肿瘤细胞的杀灭。

Objective To investigate the effect of transarterial infusion （TAI） chemotherapy on estrogen receptor （ER） and progesterone receptors （PR） expression in breast carcinoma tissue. Methods Samples of 81 patients with breast carcinoma were selected in this study. ER and PR mRNAs levels in the samples were determined by RT-PCR. Immunohistochemistry was employed to measure ER and PR expressions in different samples before and after TAI chemotherapy. Western blotting was used to assay ER and PR proteins level in the samples before and after the chemotherapy. Results RT-PCR results indicated that ER mRNA level was increased in 43 cases （53.1%） and PR level in 33 cases （40.7%）. Meanwhile, both ER and PR mRNAs levels were increased in 25 eases （30.9%）. Immunohistochemistry results showed that ER level was up-regulated in 35cases （43.2%）, and PR level in 21 cases （26.0%）. Both ER level and PR level were up-regulated in 16 out of 81 eases（ 19.8% ）. Western blotting also suggested that the both proteins levels of ER and PR were up-regulated in some patients after the chemotherapy. Conclusion Transarterial infusion neoadjuvant chemotherapy affects the mRNA and protein level of the hormone receptors, which facilitates the subsequent killing effect of chemotherapy on tumor cells. ER and PR expressions should be determined after the transarterial infusion chemotherapy, which might provide a guide for the subsequent treatment and prognosis.