c—cbl在急性非淋巴细胞白血病细胞中的表达及临床意义

Expression of c-cbl Gene in Acute Non-lymphocytic Leukemia Cells and Its Clinical Significance

目的探讨急性非淋巴细胞白血病（ANLL）细胞ccbl基因表达及临床意义。方法采用逆转录-聚合酶链反应（RT—PCR）检测47例ANLL患者ccbl基因mRNA表达水平，以20名健康人为正常对照。结果c—cblmRNA在初发ANLL患者中的表达较正常对照组明显降低（P〈0．05）；治疗缓解后表达明显升高（P〈0．05）。在初治ANLL患者中，ccblmRNA表达阳性的患者完全缓解率低于表达阴性的患者（P〈0．05）。结论在ANLL的细胞中。ccbl基因表达降低；c—cbl基因低表达患者治疗后的完全缓解率低，预后不良。

Objective To explore the expression of c-cbl gene in acute non-lymphocytic leukemia （ANLL） cells and its clinical significance. Methods The mRNA level of c cbl gene in 47 ANLL adult patients were measured by reverse transcription polymerase chain reaction（RT-PCR）. 20 healthy adults were selected as normal controls（NC）. Results The mRNA level of c-cbl gene in ANLL patients was significantly lower than in NC. The （R rate of patients with c cbl negative was lower than that of patients with c-cbl positive （P〈0.05）. Conclusion Over expression of c cbl gene may play a synergic role in the pathogenesis of ANLL and associates with CR rate in ANLL. It seems that low expression of c cbl gene may be used as a marker of poor prognosis in acute non lymphocytic leukemia.