脑红蛋白在内毒素脑损伤过程中的表达上调

Up-regulation of Expression of Neuroglobin in Rats with Brain Injury Induced by Endotoxin

目的探讨内毒素脑损伤模型大鼠的额叶皮质、海马组织、脑脊液(CSF)和血浆中脑红蛋白(NGB)的表达变化及其意义。方法将SD大鼠随机分为内毒素干预组和对照组,内毒素干预组向大鼠第四脑室内注射内毒素0.1mg/kg,对照组注入等剂量的生理盐水。于注射后3、6、12、24、48和72h采血,分离血清,收集CSF,并处死大鼠,留取额叶皮质和海马组织,应用ELISA、Western blot检测NGB含量,免疫组织化学法检测NGB的表达,干燥法检测鼠脑含水量。结果注射6h后,内毒素干扰组鼠脑含水量明显高于对照组,48h达峰值;额叶皮质、海马组织、CSF和血浆中NGB含量显著高于对照组,48h达峰值。结论在内毒素所致脑损伤中,NGB表达上调,且与内毒素的注入时间相关,NGB表达上调是机体内源性神经保护机制之一。

Objective To investigate the change of neuroglobin expression （NGB） in cortex of frontal lobe, hippocampus, cerebrospinal fluid （CSF） and plasma of rats with brain injury induced by endotoxin as well as its significance in clinic. Methods SD rats were randomly divided into test and control groups and injected intraventricularly with endotoxin and physiological saline respectively, each at a dosage of 0.1 mg/kg. The rats were killed 3, 6, 12, 24, 48 and 72 h after injection respectively, and their plasma, CSF, cortex of frontal lobe and hippocampus were collected for determination of NGB content by ELISA and Western blot, and for NGB expression by immunohistochemical method. The moisture content in brain of rats was determined by drying method. Results The NGB content in cortex of frontal lobe, hippocampus, CSF and plasma as well as moisture content in brain of rats in test group were significantly higher than those in control group 6 h, and reached peak values 48 h after injection. Conclusion The expression of NGB was up-regulated in the rats with brain injury induced by endotoxin, which was related to the time after injection with endotoxin. The up-regulation of NGB expression was one of the protective mechanisms of endogenous nerve.