重症肌无力患者胸腺组织核胞浆转运蛋白α-5的表达研究

The expression of karyopherin alpha-5 in the thymus tissue of the patients with myasthenia gravis.

目的探讨核胞浆转运蛋白α-5(karyopherin alpha-5,KPNA5)在重症肌无力(myasthenia gravis,MG)患者胸腺组织中的表达情况。方法分别利用荧光定量聚合酶链反应(fluorescence quantitative polymerase chain reaction,FQ-PCR)和免疫组化染色方法,检测MG组20例患者与对照组10名受试者胸腺组织中KPNA 5 mRNA和蛋白分子的表达。结果MG组和对照组胸腺组织中,KPNA 5 mRNA的表达水平分别为(0.71±0.08)和(0.57±0.02),两者之间的差异有统计学意义(P<0.05);KPNA 5蛋白阳性细胞均数分别为(8.99±0.49)和(5.88±0.69),差异有统计学意义(P<0.05)。结论KPNA 5分子在MG胸腺组织中表达增高,可能参与了MG的发生,是MG相关基因。

Objective To study the expression of KPNA 5 in the thymus tissues of the patients with myasthenia gravis. Methods The expressions of KPNA 5 mRNA and protein in the thymus tissue of MG and the normal control were detected using fluorescent quantitative Polymerase Chain Reaction （FQ-PCR） and immunohistochemical SP method. Resuits The expression of KPNA 5 gene at mRNA level in the thymus tissues of MG （ n = 20,0. 71±0. 08 ） was significantly higher than that of the control group （ n = 10,0. 57±0. 02） （ P 〈 0. 05 ）. The number of KPNA 5 positive cells in the thymus tissues of MG （ n = 18,8.99±0. 49） was remarkably higher than that of the control group （ n = 10,5.88±0. 69） （ P 〈 0. 05 ）. Conclusions There is high expression of KPNA 5 in the tissue of MG patients which may be associated with the generation and development of MG.