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灰色葡萄孢霉产漆酶性质的研究及其对白藜芦醇的催化 被引量:2

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摘要 目的研究灰色葡萄孢霉在生长过程中合成的一种漆酶的部分酶学性质,该漆酶能催化白藜芦醇聚合生成其脱氢二聚体viniferin,此二聚体具有比白藜芦醇更高的生物学活性。方法通过培养灰色葡萄孢霉获得该漆酶的粗酶液,测定其最适pH及温度,并用其对白藜芦醇进行催化。结果该漆酶的最适pH为4.9,最适反应温度为26℃,对20mg·L-1的白藜芦醇有较好的催化效果。结论灰色葡萄孢霉在生长过程中合成的漆酶可以催化白藜芦醇而生成viniferin。
出处 《西北药学杂志》 CAS 2009年第5期372-374,共3页 Northwest Pharmaceutical Journal
基金 上海市西部基金(2002-28) 陕西省自然科学基金(99JK122) 陕西省教育厅专项科研基金(04JK133)
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  • 1Yoshida H. Chemistry of Lacquer (Urusht) part 1[J]. J Chem Soe, 1883, 43.. 472-486.
  • 2Bertrand G. Sur la presence simultanee de la laccase et de la tyrosinase dans le suc de quelques champignons[J]. C. R. Hebd Seanees. Aead Sci, 1987, 123: 463- 465.
  • 3Laborde J. Surlacasse desvins[J]. C. R. Hebd Seances. Acad Sci, 1896, 123: 1074-1075.
  • 4康从宝,刘巧,李清心,曲音波,高培基.白腐菌产漆酶的纯化及部分酶学性质[J].中国生物化学与分子生物学报,2002,18(5):638-642. 被引量:39
  • 5许颖,兰进.真菌漆酶研究进展[J].食用菌学报,2005,12(1):57-64. 被引量:69
  • 6魏华丽,史安石,石淑兰.漆酶及其应用[J].黑龙江造纸,2004,32(4):38-39. 被引量:8
  • 7王华,尉亚辉,王庆俐,刘树文.葡萄酒中白藜芦醇的HPLC测定[J].西北农业大学学报,1999,27(4):83-87. 被引量:43
  • 8Pezet R, Gindio K. Glycosylation and oxidative dimerization of resveratrol are respectively associated to sensitivity and resistance of grapevine cuhivars to downy mildew[J]. Physiological and plant pathology, 2004, 65:297-303.
  • 9Pezet R. Purification and characterization of a 32--kDa laccase--like stilbene oxidase produced by Botrytis Cinerea [J]. FEMS Microbiol Lett, 1998, 167: 203- 208.
  • 10何为,詹怀宇,王习文,伍红.一种改进的漆酶酶活检测方法[J].华南理工大学学报(自然科学版),2003,31(12):46-50. 被引量:26

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  • 1高千千,朱启忠.漆酶-介体体系(LMS)及其应用[J].环境工程,2009,27(S1):598-602. 被引量:15
  • 2郭梅,白东清,蒲军,杜连祥,路福平.杂色云芝漆酶用于提取人参总皂甙的研究[J].食品科学,2006,27(2):166-168. 被引量:6
  • 3Jeandet P,Douillet-Breuil A C,Bessis R,et al. Phytoalexins from the vitaceae: biosynthesis, phytoalexin gene expression in transgenic plants, antifungal activity, and metabolism[J]. Journal of Agricultural and Food Chemistry, 2002,50( 10).2731-41.
  • 4Hoos G,Blaich R J. Influence of resveratrol on germination of eonidia and mycelial growth of Botrytis cinerea and Pho- mopsis viticola [J]. Journal of Phytopathology, 1990, 129 : 102-100.
  • 5Adrian M, Jeandet P, Veneau J, et al. Biological activity of resveratrol,a stilbenic compound from grapevines, against Botrytis cinerea ,the causal agent for gray mold[J]. Journal of Chemical Ecology, 1997,23 : 1689-1702.
  • 6Malacarne G, Vrhovsek U, Zulini L, et al. Resistance to Plasmopara viticola in a grapevine segregating population is associated with stilbenoid accumulation and with specific host transcriptional responses [ J ]. BMC Plant Biology, 2011,11:114.
  • 7Jang M, Cai L, Udeani G O, et al. Cancer chemopreventive activity of resveratrol, a natural product derived from grapes [J]. Science, 1997,275,218-220.
  • 8Waffo Teguo P, Krisa S, Richard T, et al. Grapevine stil- benes and their biological effects[M]//Ramawat K G, Mer- ilion J M. Bioaetive Molecules and Medicinal Plants. Heidel- berg : Springer, 2008 : 25-54.
  • 9Wan Y, Schwaninger H, He P, et al. Comparison of resist ance to powdery mildew and downy mildew in Chinese wild grapes[J]. Vitis, 2007,46 : 132- 136.
  • 10Pezet R,Gindro K,Viret O,et al. Glycosylation and oxida- tive dimerization of resveratrol are respectively associated to sensitivity and resistance of grapevine cultivars to downy mildew [J]. Physiological and Molecular Plant Pa- thology, 2004,65 : 297-303.

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