刺老苞根皮黄酮类化合物对破骨细胞分化的影响

Effect of Aralia echinocaulis Hand.Mazz flavonoids on the differentiation of osteoclast induced from bone marrow in mice

目的:研究刺老苞根皮黄酮类化合物对体外破骨细胞分化的影响。方法:建立由骨保护素配体(RANKL)和巨噬细胞集落刺激因子(M-CSF)共同细胞因子的小鼠破骨细胞骨髓诱导体系,将不同浓度的刺老苞根皮黄酮类化合物作用于破骨细胞。受试细胞分为对照组、刺老苞根皮黄酮类化合物低剂量组(10-8mol·L-1)、刺老苞根皮黄酮类化合物中剂量组(10-7mol·L-1)和刺老苞根皮黄酮类化合物高剂量组(10-6mol.L-1),并设立空白对照组。7天后取细胞玻片进行抗酒石酸酸性磷酸酶(TRAP)染色,观察破骨细胞并计数;测量抗酒石酸酸性磷酸酶(TRAP)活性以及破骨细胞表面NF-κB活化受体(RANK)mRNA表达量。结果:诱导培养的破骨细胞形态特征明显;刺老苞根皮黄酮类化合物中、高剂量组在细胞数量、TRAP活性上与对照组相比有明显统计学差异(P<0.05);刺老苞根皮黄酮类化合物各剂量组在(RANK)mRNA表达量上与对照组相比有明显统计学差异(P<0.05),且呈量效关系。结论:刺老苞根皮黄酮类化合物可以抑制体外培养的破骨细胞分化。

Objective： Effect of Aralia echinocaulis H .and. Mazz flavonoids on the differentiation of osteoclast induced from bone marrow vitro. Methods： Mononuclear cells of mice bone marrow were incubated with DMEM containing macrophage colony stimulating factor （M - CSF） and receptor activator of NF - kB ligand （RANKL）. The culture was treated with Aralia echinocaulis Hand. Mazz flavonaic/s of different concentrations （ 10^-8mol·L, 10^-7mol·L, 10^-6mol· L）. On the 7d, the culture cells were fixed and were stained for tartate- resistant acid phossphatase（TRAP）. The formarion of osteoclasts was quantified by counting the number of TRAP multinuclear cells. TRAP activity and the expression of receptor activator of NF- kB（RANK） mRNA. Results ：The appearance of reduced mice osteoclasts was typical. Compared with the control group, the number of TRAP muhinuclear cells and TRAP activity of Aralia echinocaulis Hand. Mazz flavonoids middle - dose group and high - dose group significantly decreased （ P 〈 0.05）. But the expression of RANK mRNA significantly increased （ P 〈 0.05）by treated indifferent dose Aralia echinecaulis Hand. Mazz flavonoids. Conclusion： Aralia eehinocaulis Hand. Mazz flavonoids can inhibit osteoclasts differentiation in vitro.