交联聚乙二醇二丙烯酸酯的制备及对漆酶的固定化研究

Synthesis of Cross-linked Polyethylene Glycol Diacrylates and Their Application as Supports for Laccase Immobilization

采用3种不同相对分子质量(600、6000、20000)的聚乙二醇(PEG)为单体合成了聚乙二醇二丙烯酸酯,所得产物经偶氮二异丁腈引发交联后得到具有溶胀性的固体材料,将材料作为载体通过物理吸附用于漆酶的固定化。研究了载体制备条件对固定化漆酶酶活的影响,发现交联的聚乙二醇(PEG20000)二丙烯酸酯对漆酶的固定化效果最好,适宜的交联反应条件为:交联剂质量分数0.5%,175℃反应30 min;以聚乙二醇(PEG20000)为载体进一步优化了漆酶的固定化条件;2,4-二氯酚的降解实验表明,固定化漆酶中单位质量酶的降解能力要远远高于游离漆酶。

Cross-linked polyethylene glycol diacrylates （PEGDA） were prepared with polyethylene glycols （PEG） of three different molecular weight （600, 6000 and 20000） as monomers, respectively. The products have three dimensional networks and high swelling capacity, and they were used as the carriers for the immobilization of laccase by physical adsorption. The influence of preparation conditions on the activities of immobilized enzyme were investigated and it was found that the cross-linked PEG（20000） DA showed the best immobilization effect for laccase. The proper conditions for cross-linking are as follows： cross-linking initiator concentration 0.5% , reaction temperature 175℃ and reaction time 30 min. The immobilization conditions were further optimized with PEG （20000） DA as the carrier. The degardation experiment of 2,4-dichlorophenol demonstrated that the degradation performance of the immobilized lacase was much higher than that of free lacase.