金属离子对短梗霉多糖发酵的影晌

Study on effects of metal ions on pullulan production by Aureobasidium pullulans

研究了金属离子对出芽短梗霉产色素和产多糖的影响,并优化出有利于提高多糖产量和降低色素含量的金属离子种类。本文先用单因素实验研究了不同金属离子对出芽短梗霉产多糖和产色素的影响,然后用正交实验优化出金属离子的最佳配比。在培养基中添加4μmol/L的Ca2+、2μmol/L的Fe2+、4μmol/L的Mn2+、3μmol/L的Zn2+、1μmol/L的Fe3+有利于短梗霉发酵产多糖。而在培养基中添加4μmol/L的Ca2+、1μmol/L的Fe2+、4μmol/L的Mn2+、3μmol/L的Zn2+、3μmol/L的Fe3+,发酵产黑色素较少。

Single factor comparison experiment and orthogonal experiment were adopted to study the effects of several metal ions on pullulan production by Aureobasidium pullulans and optimize metal ion composition influencing the production of pullulan and melanin. The optimal metal ion content for promoting pullulan accumulation was composed with 4μmol/L Ca^2＋ ,2μmol/L Fe^2＋ ,4μmol/L Mn^2＋ ,3μmol/L Zn^2＋ , 1μmol/L Fe^3＋ .The optimal metal ion content for reducing the melanin was composed with 4μmol/L Ca^1＋, 1μmol/L Fe^2＋ ,4μmol/L Mn^2＋ ,3μmol/L Zn^2＋ and 3μmol/L Fe^3＋.