利用微卫星和线粒体DNA标记研究山羊群体间的遗传关系

Genetic Relationships of Goat Breeds Using Micro Satellite and Mitochondrial DNA Markers

利用微卫星和线粒体DNA分析山羊的遗传多样性及系统进化关系,用25个微卫星位点分析了安哥拉山羊、山东莱芜黑山羊、罕山白绒山羊、太行山羊及乌珠穆沁绒山羊这5个山羊品种的遗传多样性,利用共祖遗传距离的UPGMA聚类表明遗传距离和地理距离是一致的,如内蒙的罕山白绒山羊和乌珠穆沁绒山羊聚到一起。利用线粒体DNA分析安哥拉山羊、山东莱芜黑山羊、鲁北白山羊、太行山羊及乌珠穆沁绒山羊,揭示这5个山羊品种分成A和C2个支系,并进行了群体结构和群体扩张分析。通过比较2种分子标记的分析结果,发现利用微卫星来研究群体的遗传多样性及品种间的关系具有较高的准确性,而线粒体在研究群体系统进化具有一定的优势,在分析品种间关系方面可能不是理想标记。

Here, we examined the genetic diversity and phylogeographic structure of domestic goats by determining mitochondrial DNA （mtDNA） and microsatellite DNA. The genetic diversity of five goat breeds including Angora goat, Shandonglaiwu black goat, Hanshan white goat, Taihang goat and Wuzhumuqin goat was surveyed using twenty-five microsatellite loci, The UPGMA phylogenetic trees constructed with the Dcoa genetic distances between the five goat breeds supported a closer relationship between Hanshan White goat and Wuzhumuqin goat. Phylogenetic analyses revealed that there were two mtDNA lineages （A,C） identified in five goat breeds including Angora goat, Shandonglaiwu black goat, Lubei white goat, Taihang goat and Wuzhumuqin goat. Population genetic structure and population expansion were carried out. Comparison with mtDNA and mierosatellite DNA revealed that microsatellite DNA is good marker for analyzing genetic diversity and genetic structure at the breed level, while mtDNA variation seems to be a poor assay in this aspect, but mitoehondrial DNA （mtDNA） has represented the most informative genomie element for untangling the origins of domestic animals.