RAPD分子标记在苹果梨的分类地位鉴定中的应用

Application of RAPD in Identification of Systematic Status of Pingguoli Pear

苹果梨是梨属中优良的种质资源,但关于其分类地位一直存在争议,为苹果梨的分类地位提供DNA水平上的依据。利用RAPD分子标记技术,通过聚类分析和遗传相似系数分析,对其进行分类研究。结果表明:当λ=12时苹果梨均首先与其各自杂交品种聚在一起后,再与白梨系统的其他类型聚在一起;苹果梨与秋子梨系统、白梨系统、砂梨系统、西洋梨系统的代表品种间的平均欧式距离分别是6.099,5.022,5.713,6.703。认为将苹果梨归为白梨系统较为适宜。

Pingguoli pear were excellent germplasm resource with cold resistance, but the ownerships of it was not find out clearly. The objective was to find out the ownership of Pingguoli pear. Using RAPD markers, delving and clearing the genetic relationships of partly Pyrus germplasm was studied, by the existent demurral and the dendrogram. The results showed; By the existent demurral on systematic status of Pingguoli pear, the general Euclidean distances between P. Bretschneideri Rehd., P.pyrifolia Nakai, P. ussuriensis Maxim, P. communis L. and Pingguoli pear were 5.022, 5.713, 6.099, and 6.703 respectively. From the dendrogram, Pingguoli pear was clustered with P. Bretschneideri Rehd. That was to say Pingguoli pear should be most similar to White-pear system.