摘要
采用改进CTAB法分别提取狗牙根(Cynodon dactylon)、结缕草(Zoysia spp.)、假俭草(Eremorchloa ophiuroides)3种暖季型草坪草基因组DNA。得到的DNA经核酸蛋白含量测定仪测得A260/A280值处于1.74～1.98之间、A260/A230集中处于1.74～1.99,经琼脂糖凝胶电泳检测各样品具有一条明亮主带并无拖尾现象。表明改进CTAB法提取得到的DNA完整性好,纯度高,适用于分子生物学的研究。
Genome DNA was extracted by the improved method from leaves of Cynodon dactylon（L.） Pers, Zoysia spp, Eremorchloa ophiuroides. The detection performed on a nucleic acids and protein analyzer showed that A260/A280 was situated betweenl.74-1.98,A260/A230 was ranged from 1.74 to 1.99.The extracted products had a bright band and without tailing bands which could be observed through agar gel electrophoresis. These results showed that genomic DNA obtained by optimum method which had a good integrity and high purity, and could be used in each kind of molecular biology research.
出处
《中国农学通报》
CSCD
北大核心
2009年第18期417-419,共3页
Chinese Agricultural Science Bulletin
基金
中国热带农业科学院热带作物品种资源研究所研究生基金项目"狗牙根属植物遗传多样性研究"(YJS-2008-B002)
关键词
暖季型草坪草
基因组DNA提取
CTAB
warm season turfgrass, extraction of genome DNA, improved CTAB method
