家蚕细胞基因工程人α-干扰素注射液对离体培养HL_(60),KB细胞及对小鼠体内S_(180),EAC细胞的影响

The Effects of IHIFN α on the Proliferation of Culture HL 60 , KB Cells and S 180 , EAC Cells in Mice

家蚕细胞基因工程人α－干扰素注射液（ＩＨＩＦＮ－α）具有抑制肿瘤生长的作用。对于离体培养的ＨＬ６０和ＫＢ细胞，ＩＨＩＦＮ－α有直接抑制肿瘤细胞生长的作用，且呈剂量依赖性关系。在体研究中，ＩＨＩＦＮ－α２．０×１０６ＩＵ／ｋｇ对Ｓ１８０有较明显的抑制趋向（Ｐ＜０．０１），抑瘤率达２１．０％；而对于ＥＡＣ，ＩＨＩＦＮ－α４．０×１０５ＩＵ／ｋｇ及２．０×１０６ＩＵ／ｋｇ两个剂量都无明显延长生存时间的作用；ＩＨＩＦＮ－α与５－Ｆｕ１０ｍｇ／ｋｇ合用对Ｓ１８０小鼠抑瘤率以及ＥＡＣ小鼠存活天数并无增效作用；但是５－Ｆｕ１０ｍｇ／ｋｇ与２．０×１０６ＩＵ／ｋｇＩＨＩＦＮ－α合用却能明显减小ＥＡＣ小鼠第９天的腹围（Ｐ＜０．０５，与５－Ｆｕ比较）。

Chinese silk worm gene engineering human interferon α(IHIFN α)has the inhibitory effect on the growth of tumor cells. IHIFN α can inhibit the proliferation of culture HL 60 and KB cells directly and dose dependently. In vivo , IHIFN α 2.0×10 6 IU/kg can inhibit the growth of S 180 , its inhibitory rate was 21.0%; whereas IHIFN α 4.0×10 5 IU/kg and 2.0×10 6 IU/kg had no effect on EAC cells in mice; IHIFN α hasn′t synergistic or additive effect to cy 15 mg/kg or 5 Fu 10 mg/kg in the index of inhibitory rate in S 180 mice and survival days in EAC cells; but from the index of the abdominal circumference in the 9th day, the circumference of group IHIFN α 2.0×10 6 IU/kg combined with 5 Fu 10 mg/kg was much shorter than that of group 5 Fu 10 mg/kg.