摘要
从５个属７３株菌中筛选到一株柑桔溃疡黄单孢菌Ｘ０３４，它可将苯甘氨酸甲酯和７－氨基去乙酰氧基头孢烷酸（７－ＡＤＣＡ）缩合生成头孢氨苄。进一步考察了碳源、氮源、无机离子对产酶活性的影响，对酶反应条件进行了研究，确定了酶反应的最佳条件为：ｐＨ６．５，７－ＡＤＣＡ浓度为２％，侧链与底物配比（ＰＧＭＥ／７－ＡＤＣＡ）为３比１，２５℃，反应９ｈ，转化率达７５％。
A Xanthomonas citri was selected from five genera and 73 bacterial strains It contains an enzyme which catalyzes the synthesis of cephalexin from 7 amino 3 desatoxy cephalosporanic (7 ADCA) and D α phenylglycinemethylester(PGME) The effect of different carbon sources, nitrogen sources and inorganic ion on enzyme activity were tested Through optimization experiment, the composition of medium was determined The maximal conversion was acquired at 25℃, pH 6 5, 2% 7 ADCA, PGME/7 ADCA 3/1 by orthogoral experiment
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1998年第4期311-315,共5页
Journal of China Pharmaceutical University
关键词
头孢氨苄
酶转化法
生产
X034
Cephalexin
Xanthomonas citri
Enzymatic production
