应用白芨建立门静脉高压动物模型

Construction of animal model of portal hypertension using bletilla striata as portal embolization agent

目的寻求制作门静脉高压动物模型的最佳方法，并以此为基础从事肝硬化后门静脉血液动力学基础研究。方法以3组共15只兔为动物模型，分别以0.2％、0．4％、0.8％浓度的白芨粉为栓塞材料，开腹注入兔门静脉内，手术前后行门静脉测压、血管造影及病理学检查，最长观察时间阎周.结果以0．4％浓度白芨粉制作门静脉高压模型最佳，4周后肝脏体积缩小20％，门静脉压平均升高40％，病理呈典型坏死后肝硬化表现。结论以白芨粉为栓塞材料制作肝硬化门静脉高压动物模型方法简单、效果可靠已重复性好.以不同浓度的白芨栓塞剂来控制坏死后肝纤维化的程度是本试验的一大特点,本动物模型的建立对深入研究肝硬化门静脉高压的成因及治疗措施有着重要意义。

Purpos To investigate on the ideal method of making the animal madel of portal hnyrtensioin Methods 15 rabbits were randomly divided into 3 groups the portal vein embolization was performed with 0. 2%, 0. 4% and 0. 8 % bletilla striata puwder respectively. Portal venous pressure measurement pobal venoguphy and the resected liver pathologic study were done before and after emhalization. The longest observation time was 4 weeks. Results The 0.4% bletilla striata powder wasthe ideal vascular embolization agent to make the animal model of portal hnyrtension. In the 4th week after embolization, liver size shrunk by 20%,portal pressure increased by an average of 40%, the pathological examination of the resected liver showed liver postnecrotic cirrhosis. Conclusions Using bletilla striata powder as vascular embolization agent to establish the animal model of portal hypertension is simple and effective. The extent of liver post necrotic cirrhosis is related to the concentration of bletilla striata powder. This study is helpful to explore the cause, blood flow dynamics and treatment of portal hypertension caused by liver cirrhosis.