摘要
为了提高丁型肝炎病毒的实验室诊断水平,建立一个高敏感性和特异性的丁型肝炎病毒的检测方法。根据GENBANK中HDV的核酸序列设计出巢式引物,采用逆转录巢式PCR方法对40例肝炎患者进行检测。结果:20例丁型肝炎病毒抗原阳性的HDVRNA均为阳性,10例丁型肝炎病毒抗体阳性中HDVRNA阳性6例,10例单纯HBsAg、HBeAg和抗HBc阳性的均未检出HDVRNA。
In order to improve the diagnostic rate of hepatitis D virus (HDV),a highly sensitive and specific method has been established in which a nested primer was designed according to HDV sequences from Genbank.Forty serum samples from patients with hepatitis were examined by using the reverse transcription nested polymerase chain reaction (RT nested PCR).The results revealed that HDV RNA was detected in 20/20 of the positive serum samples for HDAg and 6/10 of the positive samples for anti HD(negative HDAg),while HDV RNA was negative in 10 positive samples for HBsAg,HBeAg and Anti HBc.It is concluded that RT nested PCR may be useful in routine detection of HDV RNA.
出处
《河南医科大学学报》
1998年第3期97-99,共3页
Journal of Henan Medical University
关键词
丁型肝炎病毒
聚合酶链反应
逆转录
RNA
hepatitis D virus
polymerase chain reaction
reverse transcription