DGGE分析东江流域农村饮用水源中微生物多样性及其与环境因子相关性

Changes in Bacterial Community Structure of Drinking Water in Dongjiang River Valley,Determined by Denaturing Gradient Gel Electrophoresis Fingerprint and Its Relationships with Environment Factors

为评价东江流域农村饮用水源中微生物多样性及其与环境因子的相关性,分别采集了集中式供水井、塘坝型水井、猪场附近水井、普通村落水井、水库水5种水样,进行基因组总DNA的提取和主要理化指标的测定,运用DGGE技术分析各水样总DNA的PCR产物。UPGMA聚类分析DGGE指纹图谱结果表明,相同类型水样的微生物群落结构相似性较高,聚集到一个分支上;典型相关性分析(CCA)结果表明,水体中总磷(TP)和总氮(TN)的浓度与微生物群落结构的关联度最高,即磷和氮两种生命过程的基本元素对微生物群落影响最大;序列分析表明农村饮用水源中微生物群落结构丰富,包含了螺旋体门(Spirochaetes)、蓝藻门(Cyanobacteria)、变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、酸杆菌门(Acidobacteria)5个门的细菌,且每类水样拥有各自的优势菌。

In order to study the correlation between microbial diversity and the pollution degrees of the rural drinking water in Dongjiang River basin. Five types of drinking water of this basin were collected, and fifteen water samples of five types of drinking water of this basin had been collected from reservoir, central- ized water supply wells, wells in the vicinity of pig farms, wells nearby embankment and wells in villages. The six （physical, chemical, and biological） property indices of water samples were tested, at the same time, the DGGE analysis was done. The results of PCR-DGGE fingerprint indicated that bacterial richness of these drinking water samples were high, and different samples in fingerprint were different distinctively. The UPGMA dendrogram of sample basis on DGGE fingerprints showed the structure of different types of bacteria in drinking water in rural communities is obvious differences. And the results of CCA showed that the concentration of phosphorous has the largest relevance to the community structure of bacteria in water samples, followed by the concentration of nitrogen in the water. Ten typical bands were excised and sequenced. The sequences obtained were affiliated with Spirochaetes, Cyanobacteria, Proteobacteria, Actinobacteria, Acidobacteria.