具有群体感应抑制活性海洋放线菌的分离和鉴定

Isolation and Identification of Marine Actinomycetes with Quorum Sensing Inhibitory Activity

群体感应系统在许多致病菌的致病过程中发挥了重要的作用,可作为开发新型抗菌药物的理想的靶标,筛选高效的群体感应抑制剂有望成为解决细菌耐药问题的一个有效途径。我们从胶州湾海泥中分离得到放线菌47株,其发酵提取物经紫色杆菌CV026模型筛选后,发现放线菌WA-7提取物具有群体感应抑制活性,且在有效浓度时对细菌的生长没有影响。16S rDNA分析表明WA-7应属于Streptomyces属。进一步研究表明,WA-7提取物能够显著降低紫色杆菌受群体感应调节的紫色菌素产量和相关蛋白酶的表达量,且呈浓度依赖性。

Quorum sensing systems of pathogens are central regulators for the expression of virulence factors. Increasing evidence implies that targeting the quorum sensing system of many pathogenic bacteria is a promising therapeutic approach to control infections. In this work, we isolated 47 strains of actinomycetes from the mud sample of Jiaozhou Bay. Quorum sensing inhibitory activity was monitored by Chromobacterium violaceum CV026. As a result, the culture broth extract of actinomycetes WA-7 was found to have significant quorum sensing inhibitory activity. This strain was assigned to the genus Streptomyces based on its 16S rDNA sequence. Further investigation revealed that the extract could inhibit the quorum sensing-controlled violacein and proteases production of C. violaceum in a concentration-dependent manner.