基于DNA分子标记技术研究的10个特色茶花品种遗传差异分析

Genetic Diversities among 10 Representative Camellia Cultivars Based on DNA Molecular Marker

采用随机扩增多态性DNA(RAPD)技术对10个温州特色茶花品种的遗传多样性进行研究。15条十聚体随机引物共检测到105个清晰扩增位点,其中多态性位点79个,多态位点百分率为77.54%。遗传相似性分析表明:10个样品遗传距离在0.2236～0.4777之间,其中样品大朱砂与快乐王遗传距离最大(0.4905),而金盘荔枝与快乐王最小(0.2236);聚类结果显示:在遗传相似系数约为0.62时,10个茶花品种共分三组,其中样品弁天神乐、点雪分别自成一组,其余样品聚类一起。

Genetic diversities among 10 representative Camellia cultivars in Wenzhou were investigated by RAPD technology （random amplification polymorphic DNA）. The results indicated that 105 bands were produced with 15 random primers, and the genetically polymorphic bands numbered 79 accounting for 77.54%. Genetic distance analysis represented genetic distances among 10 samples ranged from 0.2236 to 0.4777 with the biggest value between samples DaZhuS and Gay Chielftain and the lowest between JinPanLZ and Gay Chielftain. Dendrogram by UPGMA software disclosed that 3 groups were produced at the similarity coefficient of 0.62, with sample Benten-kagura and DianXue isolated and the rest clustered together.