咪唑并[5,6-f][1,10]邻菲咯啉-铜(Ⅱ)-L-亮氨酸(L-酪氨酸)配合物与DNA的作用

The DNA Binding and Cleavage Properties of Cu(Ⅱ) Complexes with Imidazo-[4,5-f][1,10] phenanthroline and L-Leucinate (L-Tryptophanate)

应用电子吸收光谱、溴化乙锭(EB)荧光猝灭光谱、粘度测定及琼脂糖凝胶电泳等方法研究了配合物[Cu(IP)(L-Leu)(H2O)]ClO4(1)和[Cu(IP)(L-Trp)(H2O)]ClO4.1.5H2O(2)(IP=咪唑并[5,6-f][1,10]邻菲咯啉,L-Leu=L-亮氨酸,L-Trp=L-酪氨酸)与DNA的相互作用。分析结果表明,配合物通过插入模式与DNA作用,在还原剂维生素C存在的条件下通过羟基自由基机理对DNA进行切割,作用大小次序为:配合物1>2。

The interaction of Cu（Ⅱ） complexes [Cu（IP）（L-Leu）（H2O）]ClO4 （1） and [Cu（IP）（L-Trp）（H2O）]ClO4·1.5H2O （2） （IP=Imidazo-[4,5-f][1,10]phenanthroline,L-Leu =L-Leucinate,L-Trp =L-Tryptophanate） with DNA has been investigated by electronic absorption spectroscopy,ethidium bromide（EB） fluorescence spectroscopy,viscosity and gel electrophoresis measurements. The results indicated that the complexes interacted with DNA through intercalation,and had effective oxidative cleavage activities to pBR322 DNA in the presence of the reducing agent vitamin C involving hydroxyl radical （OH） species, as evidenced from the control data showing inhibition of DNA cleavage in the presence of OH radical quencher,viz. DMSO. The order of the DNA-binding and cleavage abilities of the complexes is complex 1 〉 2.