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血液中阿片类化合物及其代谢物的LC-M/SMS定性定量分析方法研究 被引量：1

Qualitative and quantitative analysis of opiates of abuse in blood with LC-MS-MS
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摘要 目的:建立同时测定血液中海洛因、O6-单乙酰吗啡、乙酰可待因、吗啡、可待因、吗啡-3-葡萄糖苷(M3G)和吗啡-6-葡萄糖苷(M6G)的LC-MS-MS方法。乙酰可待因是吸食非法生产海洛因的标记物,O6-单乙酰吗啡是吸食海洛因的标记物。方法:LC-MS-MS分析使用一台带电子喷雾接口的SCIEX API 2000 MS-MS仪器,样品的预处理包括使用混合型吸附柱(MCX Oasis)进行固相萃取,乙基吗啡作内标。结果:本法至少在5000 ng/ml以下呈线性关系,乙酰可待因、O6-单乙酰吗啡、可待因、吗啡的定量限各自为50、50、50、250 ng/ml,检测限各自为4,12.5,17,52 ng/ml。结论:本法灵敏度高,操作容易、适合筛选血液样品。 Objective：To develop a LC-MS-MS method for the simultaneous determination of heroin,6-acetylmorphine,acetylcodeine,morphine,codeine,morphine-3 and 6-glucuronide.Acetylcodeine was used as a marker for illicit heroin.6-Acetylmorphine was used as marker for heroin.Methods：The LC-MS-MS analysis was performed on a SCIEX API 2000 MS-MS instrument equipped with an electrospray interface.The sample pre-treatment consisted of solid phase extraction using mixed mode sorbent columns（MCX Oasis）.The ethylmorphine was us...
作者 张建新 孟品佳 张大明 张文芳 陈存仪
机构地区 公安部禁毒情报技术中心 中国人民公安大学 北京市公安局法医检验鉴定中心
出处 《中国卫生检验杂志》 CAS 2009年第9期1949-1951,共3页 Chinese Journal of Health Laboratory Technology
基金 国家科技部十一五攻关项目(2006BAK09B06)
关键词 LC-MS-MS 血液 毒品滥用 阿片 LC-MS-MS Blood Drugs of abuse Opiates
分类号 R446.11 [医药卫生—诊断学] R996 [医药卫生—毒理学]
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参考文献11

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  • 2Rook EJ, Hillebrand MJX, Rosing H, et al. The quantitative analysis of heroin, methadone and their metabolites and the simultaneous detection of cocaine, acetylcodeine and their metabolites in human plasma by high - performance liquid chromatography coupled with tandem mass spectrometry[J]. J Chromatogr B,2005,824:213 -221.
  • 3Show WZ, Pelzer M, AddisonT, et al. An automatic 96 - well solid phase extraction and liquid chromatography - tandem mass spectrometry method for the analysis of morphine, morphine - 3 - glucuronide and morphine - 6 - 6glucuronide in human plasma [ J ]. J Pharm Biomed Anal,2002, 27:143 - 152.
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中国卫生检验杂志
2009年 第9期

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