高效液相色谱法同时测定全血中7种杀鼠剂

Determination of seven rodenticides in whole blood by high performance liquid chromatography

目的：建立同时测定全血中杀鼠灵、杀鼠醚、敌鼠、氯敌鼠、溴敌隆、氟鼠灵、溴鼠灵7种杀鼠剂的高效液相色谱方法。方法：全血样品经乙酸乙酯提取后,以甲醇-0.3%乙酸铵水溶液（82：18,v/v）为流动相,经ODS-3色谱柱（250 mm×4.6 mm i.d,5μm）分离,在波长310 nm处检测。结果：分离测定过程在15 min内完成,各杀鼠剂在线性范围内,浓度与峰面积呈良好的线性关系（r=0.999）;两个浓度水平的样品加标回收率分别为91.2%-108.5%和79.2%-93.2%（杀鼠醚分别为65.8%和53.9%）;方法检出限（0.1-0.2）μg/ml;相对标准偏差（RSD）均小于3.0%。结论：方法快速、简便、准确,适用于中毒病人的中毒诊断检测。

Objective：To establish a HPLC method for the determination of warfarin,coumatetralyl,diphacinone,chlorophacinone, bromadiolone, flocoumafen, brodifacoum in whole blood. Methods：The seven rodenticides in whole blood were extracted by ethylacetate, separateded on ODS - 3 column （250 mm × 4. 6 mm i. d, 5 μm） by using the mobile phase consisting of methol - 0. 3 % ammonium acetate aqueous solution （ 82 ： 18, v/v）, detected at 310 nm. Results： The time of separation was 15 minutes. The calibration curve was linear in the concentration （r = 0. 999） and the recoveries in two levels was 91.2% - 108.5% and 79. 2% -93.2% （coumatetralyl：65.8% and 53.9% ）, detection limit was（0. 1 -0. 2） μg/ml, RSD was less than 3.0%. Condution： The method is fast, simple and accurate. It can be used in diagnosis of the clinical poisoned patients.