摘要
目的:对本地区食物中毒的沙门菌的分子分型及耐药性进行分析。方法:菌株分离、生化鉴定和血清型确定参照GB/T4789.4-2003方法进行,采用PFGE进行分子分型,利用药敏卡通过仪器对菌株进行药敏试验。结果:从12份标本中,检出8株塔克松尼沙门菌,经PFGE分型,8株源于食物中毒病人和食物标本的塔克松尼沙门菌其带型一致,为相同菌株。对8株塔克松尼沙门菌进行了21种抗生素的药敏试验,受试菌对大多数常用的头孢类和喹酮类抗生素敏感。结论:PFGE是一种非常有效的分子分型方法,建立细菌PFGE指纹图谱数据库及PulseNet,对于控制食源性疾病非常重要。
Objective：To study the molecular typing and antimicrobial resistance of Salmonella separated from food poisoning of Zhaoqing. Methods： Strain isolation, biochemical and serotype identification were done according to GB/T4789.4 - 2003 methods. Use PFGE to identify the molecular type, use antimicrobial - susceptibility card and instrument to survey antimicrobial resitance. Results：8 strains of S. taksony were found in 12 specimens from patient and food. The belt - line types of the eight S. taksony strains were the same by PFGE molecular typing,that＇s indicated that the 8 taksony came from the same sources. The result of antimicrobial test for 21 kinds of antibiotics indicated that the strains were susceptible to most of the cephalosperins and quinolones. Conclusion：PFGE is an effective molecular method for typing. It is important to establish PFGE fingerprinting data- base and PulseNet for preventing the outbreaks of foodborne disease.
出处
《中国卫生检验杂志》
CAS
2009年第9期2039-2041,共3页
Chinese Journal of Health Laboratory Technology
关键词
食物中毒
沙门菌
PFGE
耐药性
Food poisoning
Salmonella
PFGE
Antimicrobial resistance
