摘要
Low energy ion beam implantation was applied to the maize （Zea mays L） embryo proteome using two-dimensional gel electrophoresis. Protein profile analysis detected more than Ii00 protein spots, 72 of which were determined to be expressed differently in the treated and control （not exposed to ion beam implantation） embryos. Of the 72 protein spots, 53 were up- regulated in the control and 19 were more abundantly expressed in the ion beam-treated embryos. The spots of up- or down-regulated proteins were identified by matrix assisted laser desorption /ionization-time of flight mass spectrometry （MALDI-TOF-MS）. Among the identified proteins, ii were up-regulated in the treated embryos. Four of these up-regulated proteins were antioxidant molecules, three were related to stress response, two to sugar metabolism and two were associated with heat shock response. Of the five proteins up-regulated in the control embryos, three were functionally related to carbohydrate metabolism; the functions of the remaining two proteins were unknown. The data collected during this study indicate that treatment of maize embryos with low energy ion beam implantation induces changes in stress tolerance enzymes/proteins, possibly as a result of alterations in metabolism.
Low energy ion beam implantation was applied to the maize （Zea mays L） embryo proteome using two-dimensional gel electrophoresis. Protein profile analysis detected more than Ii00 protein spots, 72 of which were determined to be expressed differently in the treated and control （not exposed to ion beam implantation） embryos. Of the 72 protein spots, 53 were up- regulated in the control and 19 were more abundantly expressed in the ion beam-treated embryos. The spots of up- or down-regulated proteins were identified by matrix assisted laser desorption /ionization-time of flight mass spectrometry （MALDI-TOF-MS）. Among the identified proteins, ii were up-regulated in the treated embryos. Four of these up-regulated proteins were antioxidant molecules, three were related to stress response, two to sugar metabolism and two were associated with heat shock response. Of the five proteins up-regulated in the control embryos, three were functionally related to carbohydrate metabolism; the functions of the remaining two proteins were unknown. The data collected during this study indicate that treatment of maize embryos with low energy ion beam implantation induces changes in stress tolerance enzymes/proteins, possibly as a result of alterations in metabolism.
基金
supported by the National High-technology Project of China (2006AA100103)
the Key Project of Henan Province of China (0620010200)
