H5N2亚型禽流感病毒中药佐剂灭活苗对公雏鸡外周血T淋巴细胞亚群的影响

Effect of Inactivated Chinese Herbs Adjuvant Vaccines against H5N2 Subtype AIV on Peripheral Blood T Lymphocyte Subgroup in Chickling

将200只海兰褐公雏鸡分为4组,每组50只。A组注射H5N2亚型禽流感病毒黄芪多糖油乳剂灭活苗;B组注射H5N2亚型禽流感病毒人参皂苷油乳剂灭活苗;C组注射H5N2亚型禽流感病毒普通油乳剂灭活苗;D组注射生理盐水油乳剂(空白对照)。分别在免疫前随机选取5只鸡心脏采血,EDTA抗凝。于免疫后5、12、19 d每组每次分别随机选取5只鸡心脏采血,EDTA抗凝,检查各阶段各组鸡外周血T淋巴细胞亚群变化情况。结果表明,免疫后第12天,黄芪多糖和人参皂苷可提高CD4+/CD8+淋巴细胞的比值,与普通疫苗和对照组相比,差异极显著(P<0.01);黄芪多糖和人参皂苷可提高CD4+淋巴细胞亚群的百分率,与免疫前相比,差异极显著或显著(P<0.01或P<0.05)。

200 male Hyline Brown chicklings were divided into 4 groups（A, B,C and D）, each group with 50 broilers. The group A was injected with inactivated oil-emulsion vaccines against H5N2 subtype avian influenza virus with adjuvant of astragalus polysaccharides （APS）, the group B was injected with inactivated oil-emulsion vaccines against HSN2 subtype avian influenza virus with adjuvant of ginseng saponin （GS）, the group C and D were injected with inactivated oil-emulsion vaccines against H5N2 subtype avian influenza virus and physiological saline（control）, respectively. Five chicks were selected randomly before vaccination, the blood was taken by heart puncture and anticoagulated by EDTA. The other, each of five chicks was selected randomly from each group on the 5th, 12th and 19th days after vaccination, and the blood was also taken by heart puncture and antieoagulated by EDTA. Detecting the changes of peripheral blood T lymphocyte subgroup of each group in different stage. The data showed that the APS and GS can enhance the ratio of CD4^＋/CD8^＋ significantly compared with the common vaccine and blank groups（P〈0.01）, the APS and GS can also elevate the percentage of CD4^＋ subgroup, and the elevation rates were significantly higher than preimrnune （P〈0.01 or P〈0. 05）.