黄孢原毛平革菌低温产酶条件优化及酶学性质研究

Research on Phanerochaete Chrysosprium in Low Temperature Conditions Product Enzymes and Identity Study

以酶活力为评价指标，在10℃对黄抱原毛平革菌产酶条件进行优化，并对其部分酶作用特性进行了研究．结果：该菌在10℃下产LiP、MnP和Lac的最佳碳源是葡萄糖，最佳氮源是牛肉膏，最佳培养时间是72～108h．LiP、MnP和Lac的最适反应pH范围均为4．4～4．8，最适底物浓度是0．8～1．2mmol／L；金属离子Cu2＋，Ca2＋对LiP、MnP和Lac有激活作用．Fe2＋对三种酶活有一定的抑制作用；而Na＋则完全抑制LiP的活性，Zn2＋、Mg2＋对三种酶活影响不大．

The study enzyme vitality indicators for the evaluation through off enzyme optimization in the 10℃ conditions, and their role identity of enzymes studied. Results： Phanerochaete ehrysosprium off ligninase, manganese peroxidase and Laecase conditions is the best; the best net is glucose （net） ; Beef is the best source of nitrogen ointment; The best time is 72 - 108 hours of training. Ligninase, manganese peroxidase and Laeease are the optimal pH range 4.4 - 4.8, optimal concentrations of chemicals is 0.8 - 1. 2retool/L, Metal ions Cu2 ＋, Ca2 ＋ to ligninase, manganese peroxidase and Laecase have brought along role, Fe2 ＋ vitality of the three enzymes have certain effect, and Na ＋ the active enzyme is completely curb painting, Zn2 ＋ ,Mg2 ＋ little impact on the vitality of the three enzymes.