期刊文献+

一氧化氮和一氧化氮合酶在兔斯氏艾美耳球虫感染时作用的研究 被引量:1

THE STUDY OF NITRIC OXIDE AND NITRIC OXIDE SYNTHASE' EFFECTS ON RABBITS INFECTED WITH EIMERIA STIEDAI
下载PDF
导出
摘要 本试验以腹腔注射的途径给予斯氏艾美耳球虫(E.stiedai)感染家兔不同浓度的一氧化氮合酶(NOS)底物L-精氨酸(L-Arg)和诱导型一氧化氮合酶(iNOS)抑制剂L-氨基胍(L-AG),对家兔球虫感染时血清中NO浓度和肝的iNOS活性及各试验组家兔的病变进行了研究。试验结果表明:家兔感染E.stiedai后,血清中NO浓度和肝匀浆的iNOS活性逐渐升高,感染对照组、添加L-Arg组和添加L-AG组3组均在感染后第12 d达到最高值,而后逐渐下降,于23 d左右下降到感染前的水平;L-Arg可增强肝的iNOS的活性,促进体内大量生成NO,产生对球虫的抑制或杀伤作用,减轻球虫感染引起的病变;而L-AG则抑制肝的iNOS活性,使体内生成的NO减少,对球虫的抑制作用减弱甚至消失,从而加重球虫感染引起的病变。结果提示:NO和iNOS参与了球虫感染过程,证明NO是抗E.stiedai感染的一种效应分子。 To study changes of the activity of iNOS in the liver and the concentration of NO in blood serum and NO' effect on inhibiting or killing coccidia, rabbits infected with Eimeria stiedai were injected with different concentrations of the substrate of NOS--L--Arg and the inhibitor of iNOS--L--AG through the abdominal cavity. Then study the activity of iNOS in the liver, the concentration of NO in blood serum, the number of coccidia oocysts per gram of faeces (OPG), the anatomical and microscopical pathological changes of rabbits infected with Eimeria stiedai. The results show that when rabbits are infected with Eimeria stiedai, the activity of iNOS in liver homogenate and the concentration of NO in hood serum gradually increase and they both reach top at the twelfth day after infection in control group , L - Arg and L - AG groups. Then they graduall level before infection at about the 23th day. L - Arg could enhance the activity of iNOS in the y decrease to the liver and promote the production of NO so as to inhibit or kill coccidia and weaken the pathological changes caused by them ; on the contrary, L - AG can inhibit the activity of iNOS and reduce the production of NO, so the effect on inhibiting or killing coccidia is weakened or even disappears, thus aggravating the pathological changes caused by coccidia. The results indicate that NO and iNOS indeed participate in the course of coccidia infection, and NO plays a role in inhibiting or killing rabbit coccidia.
出处 《山东农业大学学报(自然科学版)》 CSCD 北大核心 2009年第4期503-507,共5页 Journal of Shandong Agricultural University:Natural Science Edition
基金 山东省自然科学基金资助项目(Y2003D04)
关键词 一氧化氮 诱导型一氧化氮合酶 斯氏艾美耳球虫(E.stiedai) Nitric oxide (NO) induced nitric oxide synthase (iNOS) Eimeria stiedai
  • 相关文献

参考文献10

  • 1王丙云,朱祖康,陈龙,顾有方,J.GanzalezGallego,毛鑫智.实验感染肝片吸虫山羊血浆中一氧化氮和肿瘤坏死因子的动态变化[J].中国兽医科技,2000,30(2):9-12. 被引量:6
  • 2Bohne W, Hessemann J, Gross U. Reduced replication of toxoplasma gondii is necessary for induction of brady - zoite - specific antigens : a possible role for nitric oxide in triggering stage conversion [ J]. Infect Immunol, 1994, 62 (5) : 1761 - 1767.
  • 3Stevenson M M, Tam MF, Wolf SF, et al. U -12 -induced protection against blood- stage plasmodium chabaudi as requires IFN -r and TNF -aand occursvia a nitric oxide- depent mechanism[ J]. J Immunol, 1995,155:2545 -2556.
  • 4Rajan T V, Porte P, Yates JA, et al. Role of nitric oxide in host defence against an extracelluar meta -zoan parasite, Brugia malayi[J]. Infection and Immunity, 1996, 64(8) : 3351 -3353.
  • 5Palmer R M J, Ashton D S, Moncada S. Vascular endothelial cell synthesize nitric oxide from Larginine[ J]. Nature, 1988, 333 (16) : 664 - 666.
  • 6熊静波.组织器官及培养细胞一氧化氮合酶活性测定[J].国外医学(临床生物化学与检验学分册),1997,18(4):151-153. 被引量:6
  • 7Matjos G F, Froyman R, Kennedy T. Coccidiosis control by administering toltrazuril in the drinking water fora 2 - day period [ J ]. Veterinary Parasitology ,2004,12 : 1 - 9.
  • 8李佩国,李蕴玉.常用抗球虫药对人工感染兔球虫病的疗效试验[J].中国养兔杂志,2000(4):4-5. 被引量:12
  • 9Allen P C. Fetterer R H. Effect of Eimeria acervulina infections on plasma L - arginine[ J]. Poultry Science, 2000, 79(10) : 1414 - 1417.
  • 10申兆菊,朱蓓蕾,蒋金书.NO在雏鸡球虫感染过程中的作用[J].畜牧兽医学报,2002,33(4):395-399. 被引量:8

二级参考文献6

共引文献20

同被引文献15

  • 1陈明勇,蒋金书,汪明.柔嫩艾美耳球虫对鸡的免疫预防研究[J].畜牧兽医学报,1992,23(4):354-355. 被引量:9
  • 2索勋,孔繁瑶,李安兴,殷佩云.斯氏艾美耳球虫子孢子的移行途径[J].畜牧兽医学报,1994,25(3):252-255. 被引量:18
  • 3Joyner L P,Norton C C.The immunity arising from eontinons lowlevel infection with Eimoria tenella[J].Parasitology,1973,67:333-340.
  • 4Kanyari P W N.Experimental infections with coccidiosis and serum antibody quantitation in two breed of goats[J].veterinary Parasitology,1988,28:11-18.
  • 5Licois D,Coudert P.Attempt to suppress immunity in rabbits immunized against aganst Eimeria intestinalis[J].Annales de Recherches Veterinaries,1980,11:273-278.
  • 6Lillehoj H S.Influence of inoculation dose,inoculation schedule,chicken age and host genetics on diseases susceptibihty and development of resistenee to Emeria tenella[J].infection Avian Diseases,1988,32:437-444.
  • 7Rose M E,Moekett P A.Antibodies to ccocidia:detection by the enzyme linked immunosorbent assay(ELISA)[J].Parasite Immunology,1953,5:429-489.
  • 8Hhillip H K,Theodore T K,John C F.Eimeria stiedai:Delayed hypersensitiyity response in rabbit coccidiosis[J].Exeperimental Parasitology.1976,39(1):59-68.
  • 9Long P L.The problem of coccidiosis:General consideration.L[A].In:Long K,Boorman N and Freeman B hi.Arian coccidiosis British Poultry Scienci Ltd[M].Edeinburgh,1978:3-28.
  • 10Long P L.Coccidia and cossidiosis[M].(2nd).Verlag Parey,Berlin 1974.

引证文献1

二级引证文献10

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部