多次给予羟乙基淀粉或高渗氯化钠溶液对实验性脑出血大鼠局部氧化损伤和炎性反应的影响

Effects of Repeated Dosing of 6% Hydroxyethyl Starch or 7.5% Sodium Chloride on Inflammatory Response and Oxidative Damage in Experimental Intracerebral Hemorrhage Rats

目的探讨多次给予羟乙基淀粉或高渗氯化钠(NaCl)溶液对实验性脑出血(ICH)大鼠脑组织局部炎性反应和氧化损伤的影响。方法清洁级雄性SD大鼠167只随机分为假手术组(S组,n=20)、ICH组(M组,n=38)、氯化钠组(N组,n=55)和羟乙基淀粉组(H组,n=54)。采用立体定向技术向大鼠右侧尾状核注入自体血50μl建立ICH模型,S组仅刺入基底节,但不注血。N组分别于造模后2h、24h、48h、72h静脉输注7.5%NaCl溶液5ml/kg,H组分别于造模后2h、24h、48h、72h静脉输注6%羟乙基淀粉(130/0.4)30ml/kg。S组和M组分别于造模后2h、24h、48h、72h随机取5只大鼠断头处死,N组和H组则在上述时间点于进行输液治疗前、后各抽取5只大鼠处死。测定血肿周围区脑组织匀浆中白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、超氧化物歧化酶(SOD)和丙二醛(MDA)的含量;观察生存情况。结果N组IL-6在24h、72h输液后高于M组(P<0.05)。H组TNF-α在24h、48h输液前低于M组(P<0.05)。M组SOD在48h、72h降至最低;N组和H组SOD在24h、48h和72h输液后均高于M组(P<0.05);H组在72h输液后MDA明显低于M组(P<0.01)。结论多次给予6%羟乙基淀粉(130/0.4)或7.5%NaCl溶液可降低实验性脑出血脑组织局部的氧化损伤,减轻炎性反应,可能具有脑保护的作用。

Objective To observe the effects of repeated dosing of 6% hydroxyethyl starch （130/0.4） or 7.5% sodium chloride on brain edema after experimental intracerebral hemorrhage （ICH） in rats. Methods 167 male SD rats were divided into four groups randomly： Sham operation group （S, n=20）, ICH control group （M, n=38）, 7.5% sodium chloride group （N, n=55） and 6% hydroxyethyl starch group （H, n= 54）. The model of the ICH was established with stereotactically infusing 50 μl of the autologous femoral artery blood into the right caudate nucleus, group N and group H received 7.5 % sodium chloride 5 ml/kg and 6 % hydroxyethyl starch 30 ml/kg at 2 h, 24 h, 48 h and 72 h after operation respectively. The tumor necrosis factor-alpha （TNF-α）, interleukin-6 （IL-6）, superoxide dismutase （SOD） and malondialdehyde （MDA） in the tissue around the hemorrhage were measured at different time point. Results The IL-6 in group N was significantly more than that in group M at 24 h and 72 h after infusion （P〈0.05）, and the TNF-α in group H was less than that in group M at 24 h and 48 h after infusion （P〈0. 05）. The SOD in group M decreased to the bottom at 48 h and 72h after ICH. SOD in group N and group H at 24 h, 48 h and 72 h after infusion was both significant more than that in group M （P〈0.05）. MDA in group H at 72 h after infusion was less than that in group M （P〈0.05）. Conclusion Repeated infusion of 6% hydroxyethyl starch （130/0.4） or 7.5 % sodium chloride can decrease inflammatory response of brain tissue after ICH, which may protect brain from oxidative damage.