HPLC测定脉络宁胶囊中肉桂酸的含量

Determination of Cinnamic Acid Content in Mailuoning Capsules by HPLC

目的：建立脉络宁胶囊中肉桂酸的含量测定方法。方法：高效液相色谱法,采用Venusil MP C18柱为分析柱,甲醇—0.1%磷酸溶液（pH3.5）（55∶45）为流动相,检测波长278 nm,流速1.0 ml/min。结果：肉桂酸在0.011－0.090μg与峰面积呈良好的线性关系（Y=103640X-8954.4,R^2=0.9998）,平均回收率为100.08%,RSD=1.32%。结论：方法具有操作简便、可靠、重现性好的特点,能准确控制药品质量。

Objective：To establish an HPLC method for the determination of Cinnamie Acid Content in Mailuoning Capsules. Methods： Venusil MP C18 （5 μm , 4.6 × 250 mm）column was used for the HPLC determination, and the mobile phase consisted of methanol and 1% phosphoric acid（ pH 3.5 ） （55 ： 45 ） at a flow rate of 1.0 ml/min. The detection wavelength was 278 nm. Results： Cinnamie Acid has good linear relationship in the range of 0.011 -- 0. 090 μg （ Y = 103640X - 8954.64, R^2 = 0. 999 8 ）. The average recovery was 100. 16 %, RSD = 1.42 %. Conclusions ： The method is rapid, accurate , good reproducibility, and suitable for quality control of the Mailuoning Capsules.