摘要
目的应用基因芯片技术研究整合素基因家族在三种肺癌细胞株A549(肺腺癌)、H1650(肺泡细胞癌)、DMS53(小细胞肺癌)中的表达差异。方法提取肺癌细胞A549、H1650、DMS53的mRNA,反转录为cDNA,以32P标记cDNA,与整合素基因芯片进行杂交,经放射自显影后读取灰度值并进行统计学分析。结果整合素基因家族中ITGB1在三种肺癌细胞A549、H1650和DMS53上均出现高表达;ITGA4在H1650的表达显著高于A549和DMS53(>2倍);ITGA3在小细胞癌细胞DMS53中的表达显著低于非小细胞癌(NSCLC)细胞A549和H1650;ITGB6、ITGB8在DMS53中的表达显著高于A549。结论一些整合素亚单位在三种肺癌细胞株A549、H1650、DMS53中的表达谱存在差异,有望为肺癌的靶基因治疗提供实验依据。
Objective To analyze the expression of integrins in 3 different lung cancer cell lines including A549 （adenocareinorna）, H1650 （hronchioloalveolar carcinoma） and DMS53 （small-cell carcinoma）. Methods The mRNA was extracted from A549, H1650 and DMS53, and then reversely transcribed to the cDNA. The mixed probes labeled by 32p were hybridized to the microarray chip, and exposed to a phosphor screen and scanned in a phosphoimager. Results ITGB1 integrin was observed with up-regula- tion in all the lung cancer cells. The expression level of several integrins including ITGA3, ITGA4, IT- GB6 and ITGB8 displayed a〉2. 0-fold difference. Up expression of ITGA4 was detectable in H1650, and ITGA3 was observed in all the lung cancer cells except in DMS53, ITGB6 and ITGB8 were detectable in DMS53. Conclusion The expression of several integrins varied in three lung cancer cells. Integrins may play an important role in lung cancer metastasis,
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2009年第9期734-736,共3页
Cancer Research on Prevention and Treatment
关键词
肺癌细胞
基因芯片
整合素
Lung cancer cells
eDNA micro arrays
Integrins
