Twist、IGF-1在大肠癌中的表达及其意义

Expression and Significance of Twist and IGF-1 in Colorectal Carcinomas

目的检测Twist、IGF-1蛋白在大肠癌中的表达,分析与临床病理学因素的关系,探讨两者在大肠癌发病机制中的相互作用以及与患者预后的关系。方法选取不同类型的大肠癌组织48例,癌旁5cm以上的正常组织24例,采用SP法检测Twist、SABC法检测IGF-1的表达。结果在大肠癌组织中Twist、IGF-1的阳性表达率分别为47.92%、64.58%,均显著高于癌旁正常组织中的12.50%、16.67%。Twist、IGF-1在大肠癌组织中的表达与肿瘤的浸润程度、淋巴结转移、Dukes分期显著相关,Twist的表达还与患者5年生存情况有显著的相关性。Twist和IGF-1在大肠癌组织中的表达呈明显相关性。结论Twist、IGF-1可能协同作用参与大肠癌的浸润、转移,同时Twist阳性表达提示预后不良,对判断大肠癌患者的预后有一定的参考价值。

Objective To investigate the expression of Twist and IGF-1 in colorectal carcinomas and the relation with clinical and pathological factors, and to analyze the interaction of Twist and IGF-1 on noso- genesis of colorectal carcinomas and the prognosis of patients. Methods Immunohistochemical SP meth- ods was adopted to examine the expression of Twist and Immunohistochemical SABC methods was adopt- ed to examine the expression of IGF-1 in colorectal carcinomas（48 cases） and paracancerous normal tis- sues（24 cases）. Results The positive rates of Twist and IGF-1 in colorectal carcinomas（47. 92% and 64.58 %） were significantly higher than those in paraeaneerous normal tissues （12. 50 % and 16. 67 % ）. The overexpression of Twist and IGF-1 were significantly related with the tumor differentiation, the depth of invasion, lymph node metastasis and the Dukes stage. The overexpression of Twist was aslo signifi- cantly related with the 5 year survival state. The expression of Twist in colorectal carcinomas was obvi- ously related with IGF-1. Conclusion The expression of Twist and IGF-1 may play a cooperative role in the infiltration and metastasis of coloreetal carcinomas. The positive expression of Twist indicates poor prognosis and provide a valuable reference for the decision of prognosis.