羊Ⅱ型肺泡上皮细胞的分离培养及鉴定

Isolation and culture of alveolar type Ⅱ cell from goat

为改进羊肺泡Ⅱ型上皮细胞的分离和培养技术,本研究采用IgG黏附法结合磁化树脂微粒法分离纯化了山羊肺泡Ⅱ型上皮细胞,碱性磷酸酶染色检查表明Ⅱ型细胞占90%以上,台盼蓝染色活细胞为95%以上。羊肺泡Ⅱ型上皮细胞分离纯化方法的建立,为今后体外分离培养绵羊肺腺瘤病毒(JSRV)的探索性工作奠定了基础,也为研究肺纤维化及肺癌发病机理等重大课题提供了重要的技术支持。

This paper described a improved isolation and cultivation of aveolar type II （AT II ） cell by depleting leukocyte through IgG and magnetic resin. The proportion of AT II cell to cell mixture is more than 90 % after staining by alkaline phosphatase （AKP） and nuclear fast red （NFR）, and the ratio of 95 % living cells was analyzed by trypan blue staining. This method may be useful in virus isolation and Jaggsiekte sheep retrovirus （JSRV） culture in vitro, and for mechanism research of pulmonary fibrosis and pathogenesis of lung cancer.