早孕期补充戊酸雌二醇对雄性子代大鼠生殖系统发育的影响

Effects of Exposure to Estradiol Valerate in Early Pregnancy on the Reproductive System Development of F1 Male Rats

目的:建立大鼠早孕期戊酸雌二醇用药模型,从肛门生殖器距离(AGD)、附睾、睾丸组织发育等方面评估戊酸雌二醇对雄性子代生殖系统发育的影响。方法:妊娠SD大鼠随机分对照组及低、中、高剂量组,孕6～15d每天分别给予戊酸雌二醇0、0.2、0.5、0.8mg/kg灌胃,分娩后正常喂养其雄性子代,出生3d、出生21d分别测量雄性子代AGD,出生60d测量附睾、睾丸的脏器系数(附睾、睾丸重量g/大鼠体重100g),进行睾丸组织学切片观察生精小管的形态学变化,测量生精小管直径和上皮高度。结果:对照组及各给药组雄性子代出生3d、出生21d的AGD比较,无明显差异(P>0.05);对照组及各给药组子代出生60d的附睾、睾丸脏器系数比较、睾丸生精小管直径及上皮高度比较,均无明显差异(P>0.05)。结论:早孕期大鼠补充一定剂量(0.2～0.8mg/kg)戊酸雌二醇,出生的雄性子代在生殖系统发育上未受明显影响,性成熟期的睾丸组织学未见明显改变。

Objective： To establish the rat model of estradiol valerate medication in early pregnancy, and to investigate the effects of estradiol valerate on the development of the reproductive system of the first filial generation （ F1 ） male rats by evaluating the anogenital distance （AGD） and the development of the testis and epididymis. Methods： Pregnant SD rats were divided at random into a blank control group and a low dose, a medium dose and a high dose medication group to receive intragastric estradiol valerate at 0.2 mg/kg, 0.5 mg/kg and 0.8 mg/kg, respectively. The newborn F1 male rats were normally fed. Their anogenital distances were measured on postnatal day （PND） 3 and 21, the organ coefficients of the testis and epididymis （ testicular and epididymal weight g/body weight 100 g） were obtained on PND 60, the morphological changes of spermatogenic cells were observed by testis biopsy, and the diameter of the seminiferous tubule and epithelial height were measured. Results： There was no significant difference between the control and medicated F1 male rats in AGD on PND 3 and 21 （ P 〉 0.05 ） , nor in the organ coefficients of the testis and epididymis on PND 60 （ P 〉 0.05 ）, nor in the diameter of the seminiferous tubule and epithelial height. Conclusion ： Medication of estradiol valerate （0.2 - 0.8 mg/kg） to rats in early pregnancy neither significantly affects the reproductive system development, nor induces obvious histological changes of the testis in the sexual maturation period of their F1 males.