JC-1单标法检测精索静脉曲张患者精子线粒体膜电位的研究

Evaluation of Sperm Mitochondrial Membrane Potential in Varicocele Patients Using JC-1 Fluorescent Staining

目的:检测精索静脉曲张患者精子线粒体膜电位并探讨其临床意义。方法:将67例精索静脉曲张患者分为VC1组(精索静脉曲张1度,n=26)、VC2组(精索静脉曲张2度,n=21)和VC3组(精索静脉曲张3度,n=20),以正常生育男性为正常对照组(n=29)。通过计算机辅助精液分析系统进行精液常规分析。精液标本洗涤处理后用荧光染料JC-1染色后上流式细胞仪分析,检测精子线粒体膜电位(JC-1+%)。结果:VC1、VC2、VC3组精子线粒体膜电位[(56.29±16.32)%,P<0.05;(45.04±13.21)%,P<0.01;(31.63±12.91)%,P<0.01]均显著低于正常对照组[(76.21±13.96)%]。96例标本中,JC-1+%与(a+b)级精子百分率呈显著正相关(r=0.693,P=0.000)。结论:精索静脉曲张可引起精子线粒体膜电位降低,可能是导致男性不育的重要原因之一。

Objective ： To detect sperm mitochondrial membrane potential （MMP） of varicocele patients and investigate its clinical significance. Methods ： Sixty-seven varicocele patients were divided into a VC1 （ grade 1, n = 26） , a VC2 （ grade 2, n = 21 ） and a VC3 group （ grade 3, n = 20）. And 29 normal fertile volunteers were included in a control group （ n = 29）. Conventional semen analyses were performed by computer-assisted semen analysis （CASA）. Semen samples were washed, followed by JC-1 staining to evaluate the sperm MMP （JC-1 ＊% ） by flow cytometry. Results ： The sperm MMPs of the VC1, VC2 and VC3 groups were siginifieanfly lower （ [56.29 ±16.321%, P 〈0.05; [45.04±13.211%, P〈0.01; [31.63 ±12.911%, P〈0.01） than that of the control （[76.21 ± 13. 96] % ）. There was a significant positive correlation between the percentage of JC-1 ＋ and that of grade （a ＋ b） sperm （ r = 0.693, P = 0. 000）. Conclusion ： The decreased MMP in the sperm of varicoeele men might be one of the important causes of male infertility.