隐孢子虫卵囊DNA纯化方法的研究进展

DNA Purification Methods of Cryptosporidium Oocysts

PCR技术不仅可检测极微量的隐孢子虫,而且具有高度特异性,在隐孢子虫检测中发挥重要作用。PCR检测中,隐孢子虫卵囊模板DNA的制备尤为重要,需对卵囊进行分离纯化和裂解后才能提取纯化DNA。本文综述目前常用的卵囊分离纯化、裂解和DNA提取纯化的方法。

PCR technique can especially detect trace amount of Cryptoporidium oocysts. The preparation of template DNA is important for PCR detection. Before DNA purification, the oocysts should be purified and splitted. This paper summarizes the ordinary purification and splitting methods for Cryptoporidium oocysts.