单侧睾丸扭转对生殖细胞凋亡及黄芪保护作用的实验研究

The influence of unilateral testicular torsion on the reproductive cell and protective role of astragalus on testicular tissue

目的观察大鼠单侧睾丸扭转/复位后患侧和对侧睾丸生精细胞凋亡情况,探讨单侧睾丸扭转/复位后生殖能力下降的机制以及黄芪注射液对其再灌注损伤的保护作用。方法将40只健康雄性Wistar大鼠分为4组,分别为假手术对照组(A组),睾丸扭转/复位组(B组),睾丸扭转/复位+单次腹腔内注射黄芪注射液组(C组)及扭转/复位+连续腹腔内注射黄芪注射液组(D组),每组10只。按Turner法建立睾丸扭转/复位模型,所有大鼠均在同等条件下喂养至术后7d处死,切取双侧睾丸后检测凋亡指数。结果扭转侧睾丸生殖细胞凋亡指数(AI)A组(5.82±1.21)与B组(36.18±8.40)、C组(20.39±3.57)、D组(11.61±5.12)相比差异有显著性(P<0.05),B组明显高于C组及D组(P<0.05),C组与D组相比差异有显著性(P<0.05);B组对侧睾丸(12.95±3.06)与C组(9.45±1.71)、D组(7.56±1.06)两组对侧睾丸AI相比差异有显著性(P<0.05),C、D两组对侧睾丸AI差异有显著性(P<0.05)。结论单侧睾丸扭转可致患侧和对侧睾丸生精细胞凋亡明显增加,黄芪注射液可明显减少双侧睾丸生殖细胞凋亡,连续应用黄芪注射液优于单次应用。

Objective To investigate rat unilateral testicular torsion resulting in ipsilateral and contralateral testicular germ cell apoptosis, and to discuss the protective mechanism of astragalus injection and decreased reproductive capacity after unilateral testicular torsion/detorsion. Methods 40 healthy adult Wistar rats were divided randomly into four groups, sham operation group （group A, n = 10）, torsion/detorsion group （group B, n = 10）, single astragalus plus detorsion group （group C, n = 10）, successive astragalus plus detorsion group （group D, n = 10）. Testicular torsion/detorsion model was established in terms of turner methods. All rats were fed under the same conditions for 7 days and sacrificed. Bilateral orchidectomy was performed and germ cell apoptosis was detected. Results AI of germ ceil： there was a significant difference among group A （5.82±1.21）, B （36.18±2.40）, C （11.61±5.12）, and D （11.61±5.12） in the torsional testis （P〈0.05）. There was a significant difference among group A （5.65±1.42）, B （12.95±3.06）, C （9.45±1.71）, D （7.56±1.06） in the contralateral testis （P 〈 0.05）. Conclusion Unilateral testicular torsion can induce ipsilateral and contralateral testicular germ cell apoptosis significantly increases. Astragalus injection can significantly reduce bilateral testicular germ cell apoptosis. Continuous application of astragalus injection is better than a single application.