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flk-1基因在胎肝造血细胞增殖中的调控作用 被引量：1

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摘要 【目的】探讨flk-1基因在胎肝造血细胞体外扩增中的调控作用。【方法】体外培养E14胎肝造血细胞,应用Western-Blot检测flk-1配基VEGF及其抑制剂SU5416对E14胎肝造血细胞flk-1蛋白表达水平的影响,通过细胞计数、氚标胸腺嘧啶脱氧核苷掺入实验及细胞周期分析观察flk-1配基VEGF及其抑制剂SU5416对E14胎肝造血细胞增殖的影响,造血集落CFU-GM(colony-forming unit-granulocyte/macrophage粒-巨噬细胞系集落形成单位)培养检测造血祖细胞的功能状态。【结果】VEGF或VEGF加SU5416均可促进胎肝造血细胞的增殖,细胞数目分别为对照组的108%和142%,并且集落数量也显著增加,分别为对照组的159%和151%(P<0.01);单独应用SU5416使造血细胞的数目较对照组减少了8%,但集落产率与对照组无显著性差异。Western blot结果经统计学分析,SU5416组和VEGF加SU5416组flk-1蛋白表达强度分别为对照组的165%(P<0.01)和57%(P<0.05),与对照组相比有显著性差异。【结论】VEGF受体flk-1和flt-1均参与介导VEGF对胎肝造血细胞增殖的调控作用。SU5416阻断flk-1信号转导可上调flk-1的蛋白表达,Flt-1活化可下调flk-1的蛋白表达。
作者 张钰 姜树晶 那晓东 陈晓勤 郑芹
机构地区 中山大学中山医学院病理生理学教研室 中山大学肿瘤防治中心
出处 《中山大学学报（医学科学版）》 CAS CSCD 北大核心 2009年第A03期1-4,共4页 Journal of Sun Yat-Sen University:Medical Sciences
基金 国家自然科学基金(No.30400225) 广东省自然科学基金(No.04300227)
关键词 胎肝造血细胞 增殖 血管内皮生长因子(Vascular ENDOTHELIAL GROWTH factor VEGF) FLK-1
分类号 R363 [医药卫生—病理学]
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参考文献9

  • 1Jin H, Xu J, Wen Z. Migratory path of definitive hematopoietic stem/progenitor cells during zebrafish development [ J ]. Blood, 2007,109 (12) : 5208 - 5214.
  • 2Kiel M J, Iwashita T,Yilmaz OH, et al. Spatial differences in hematopoiesis but not in stem cells indicate a lack of regional patterning in definitive hematopoietic stem cells [ J ]. Dev Biol, 2005,283 ( 1 ) : 29-39.
  • 3Chang KT, Sefc L, Psenak O, et al. Early fetal liver readily repopulates B lymphopoiesis in adult bone marrow [ J ]. Stem Cells, 2005,23 (2) : 230-239.
  • 4倪效,燕敏.VEGF受体功能研究进展[J].生命科学,2008,20(1):120-124. 被引量：24
  • 5Miyagi T, Takeno M, Nagafuchi H, et al. FLK1^+ cells derived from mouse embryonic stem cells reconstitute hematopoiesis in vivo in SCID mice [J]. Exp Hematol, 2002,30(10) : 1444-1453.
  • 6Hattori K, Heissig B, Wu Y, et al. Placental growth factor reconstitutes hematopoiesis by recruiting VEGFR 1 + stem cells from bone-marrow microenvironment [J]. Nature Med, 2002, 18(8):841-849.
  • 7Shalaby F, Ho J, Stanford WL, et al. A requirement for Flkl in primitive and definitive hematopoiesis and vasculogenesis [J ]. Cell, 1997,89 (6) : 981-990.
  • 8Wang D, Donner DB, Warren RS. Homeostatic modulation of cell surface KDR and Fltl expression and expression of the vascular endothelial cell growth factor (VEGF) receptor mRNAs by VEGF [J]. J Biol Chem, 2000,275(21 ) : 15905-15911.
  • 9Autiero M, Waltenberger J, Communi D, et al. Role of PlGF in the intra- and intermolecular cross talk between the VEGF receptors Fltl and Flkl [J]. Nat Med, 2003,9 (7) : 936-943.

二级参考文献37

  • 1Takahashi H, Shibuya M. The vascular endothelial growth factor (VEGF)/VEGF receptor system and its role under physiological and pathological conditions. Clin Sci. (Lond),2005, 109(3): 227-41
  • 2Suto K, Yamazaki Y, Morita T, et al Crystal structures of novel vascular endothelial growth factors (VEGF) from snake venoms: insight into selective VEGF binding to kinase insert domain containing receptor but not to fms-like tyrosine kinase-1. J Biol Chem, 2005, 280(3): 2126-31
  • 3Muller YA, Li B, Christinger HW, et al. Vascular endothelial growth factor: crystal structure and functional mapping of the kinase domain receptor binding site. Proc Natl Acad Sci USA, 1997, 94(14): 7192-7
  • 4De Falco S, Gigante B, Persico MG. Structure and function of placental growth factor. Trends Cardiovasc Med, 2002, 12(6): 241-6
  • 5Woolard J, Wang WY, Bevan HS, et al. VEGF165b, an inhibitory vascular endothelial growth factor splice variant: mechanism of action, in vivo effect on angiogenesis and endogenous protein expression. Cancer Res, 2004, 64(21): 7822-35
  • 6Lee S, Jilani SM, Nikolova GV, et al. Processing of VEGFA by matrix metalloproteinases regulates bioavailability and vascular patterning in tumors. J Cell Biol, 2005, 169(4): 681-91
  • 7Christinger HW, Fuh G, de Vos AM, et al. The crystal structure of placental growth factor in complex with domain 2 of vascular endothelial growth factor receptor-1. J Biol Chem, 2004, 279(11): 10382-8
  • 8Gallicchio M, Mitola S, Valdembri D, et al. Inhibition of vascular endothelial growth factor receptor 2-mediated endothelial cell activation by Axl tyrosine kinase receptor. Blood,2005, 105(5):1970-6
  • 9Guo DQ, Wu LW, Dunbar JD, et al. Tumor necrosis factor employs a protein-tyrosine phosphatase to inhibit activation of KDR and vascular endothelial cell growth factorinduced endothelial cell proliferation. J Biol Chem, 2000, 275(15): 11216-21
  • 10Carmeliet P, Ferreira V, Breier G, et al. Abnormal blood vessel development and lethality in embryos lacking a single VEGF allele. Nature,1996,380(6573): 435-9

共引文献23

同被引文献8

  • 1Dzierzak E,Speck NA.Of lineage and legacy:the development of mammalian hematopoietic stem cells[J].Nat Immunol,2008,9(2):129-136.
  • 2Kim I,He S,Yilmaz OH,et al.Enhanced purification of fetal liver hematopoietic stem cells using SLAM family receptors[J].Blood,2006,108(2):737-744.
  • 3Tjwa M,Luttun A,Autiero M,et al.VEGF and PlGF:two pleiotropic growth factors with distinct roles in development and homeostasis[J].Cell Tissue Res,2003,314(1):5-14.
  • 4Ratajczak MZ,Ratajczak J,Machalinski B,et al.Role of vascular endothelial growth factor(VEGF)and placenta-derived growth factor(PlGF)in regulating human haemopoietic cell growth[J].British Journal of Haematology,1998,103(4):969-979.
  • 5Gerber HP,Malik AK,Solar GP,et al.VEGF regulates haematopoietic stem cell survival by an internal autocrine loop mechanism[J].Nature,2002,417(6892):954-958.
  • 6Bagli E,Stefaniotou M,Morbidelli L,et al.Luteolin inhibits vascular endothelial growth factor-induced angiogenesis;inhibition of endothelia cell survival and proliferation by targeting phosphatidylinositol 3-kinase activity[J].Cancer Res,2004,64(21):793-796.
  • 7Autiero M,Waltenberger J,Communi D,et al.Role of PlGF in the intra-and inter molecular cross talk between the VEGF receptors Flt1 and Flk1[J].Nat Med,2003,9(7):936-943.
  • 8陈晓勤,那晓东,余伟华,李树浓,张秀明,曾有健,林承光,郑芹,蒋涛.胚胎干细胞来源造血细胞的细胞表型和功能分析[J].中山大学学报（医学科学版）,2009,30(4):367-371. 被引量：3

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中山大学学报（医学科学版）
2009年 第A03期

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