^(125)I放射性粒子对人食管癌裸鼠皮下移植瘤的抑制作用研究

The lethal effect of interstitial brachytherapy with ^(125)I seeds in treatment of the human esophageal tumor xenografts in nude mice

目的:观察125I粒子植入对人食管癌裸鼠皮下移植瘤的抑制作用。方法:采用雄性BALB/C裸小鼠建立人食管癌Eca-109细胞株的裸鼠皮下移植瘤模型,将荷瘤鼠随机分为5组(每组5只):对照组(A组)、假手术组(B组)、低剂量组(C组,植入7.4×106Bq粒子1枚)、中剂量组(D组,植入14.8×106Bq粒子1枚)和高剂量组(E组,植入29.6×106Bq粒子1枚)。第30天检测各组移植瘤体积,并观察各组移植瘤组织病理学变化。结果:C、D、E组瘤体积与A组比较显著缩小,差异均有统计学意义(P<0.05),D、E组瘤体积与C组瘤体积相比均有统计学意义(均P<0.05),但D、E组之间瘤体积相比差异无统计学意义(P>0.05)。B组和A组瘤体积相比差异无统计学意义(P>0.05),C、D、E植入后30d肿瘤体积抑瘤率分别为71.5%、90.8%、92.1%。结论:125I粒子对人食管癌裸鼠移植瘤具有一定的抑制和杀伤作用。

Objective：To observe the lethal effect of interstitial implantation with ^125I seeds in nude mice with the human esophageal tumor Eca - 109 cell. Methods： The animal models of the human esophageal Eca- 109 cell tumor were established through the injection of Eca - 109 ceils into nude mice subcutaneously, following by the implantation of the ^125 I seeds into the tumor mass. The animals were divided into 5 groups and 5 nude mice in each group ： A ： control group, B ： sham group. C ： low - close group （ treated with 7.4 × 10^6 Bq ^125I seed）. D ： middle - dose group （ treated with 14.8 × 10^6 Bq ^125I seed）. E： high - dose group （treated with 29.6 × 10^6 ^125I seed）. The volume of tumor was measured, the inhibitory rate was calculated and the histopathological changes were observed at the 30th day. Results： The volume of tumor in C, D, E group was statistically significant different to A group. The volume of tumor in D, E group was statistically significant different to C group. But the volume of tumor between D and E groups did not demonstrate a statistical difference. The inhibitary rates of C, D, E group were 71.5% ,90.8% and 92.1% respectively. There was statistically significant different in the inhibitory rate between D, E and C group. Conclusion： This study verified that the ^125I radioactive seed shows a positive inhibiting effect to the Eca- 109 cell tumor xenografts in nude mice.