期刊文献+

GnRH-Ⅱ对子宫内膜异位症患者离体培养的子宫内膜间质细胞分泌VEGF的影响 被引量:3

Effect of GnRH-Ⅱ on VEGF secreted by stromal cells from endometrium of endometriosis
下载PDF
导出
摘要 目的:检测体外培养的子宫内膜异位症(endometriosis,EMs)患者在位和异位内膜间质细胞分泌的血管内皮生长因子(vascular endothelial growth factor,VEGF)的浓度,并观察不同浓度的II型促性腺激素释放激素(GnRH-Ⅱ)对在位和异位内膜间质细胞分泌VEGF的影响。方法:分别给予原代培养的EMs患者在位与异位子宫内膜间质细胞不同浓度(1×10-10~1×10-6mol/L)的GnRH-Ⅱ处理,不加GnRH-Ⅱ组作为对照,采用酶联免疫吸附法(ELISA)测定培养液中VEGF浓度并进行比较。结果:体外培养的EMs患者异位子宫内膜间质细胞分泌VEGF,培养48 h后分泌量与在位子宫内膜间质细胞比较差异无统计学意义(P(0.05)。1×10-10~1×10-6mol/L的GnRH-Ⅱ可呈浓度依赖性地抑制体外培养的在位和异位子宫内膜间质细胞分泌VEGF(P<0.01),且对异位子宫内膜间质细胞的抑制作用强于在位(P<0.01)。结论:EMs患者的异位子宫内膜间质细胞分泌VEGF的能力与在位子宫内膜的相近,这对EMs的形成和发展可能起重要作用。GnRH-Ⅱ对EMs患者体外培养的异位子宫内膜间质细胞VEGF的分泌有明显的抑制作用,且作用明显强于对在位内膜间质细胞的。 Objective To inspect the effect of gonadotropin-releasing hormonel Ⅱ ( GnRH-Ⅱ ) on the secretion of VEGF by eutopic and eetopic endometrial stromal cells cultured in vitro. Methods Eutopic and ectopic stromal cells cultured in vitro were treated with different concentrations (1 × 10^-10 -1 × 10^-6 mol/L)of GnRH-Ⅱ ,and the control group was not treated by GnRH-Ⅱ. Enzymelinked immunosorbent assay ( ELISA ) was used to measure the VEGF protein in the medium of the above 2 groups. Results There was no difference between the VEGF protein expressed by eutopic and ectopic stromal cells in the medium after culturing in vitro for 48 hours ( P 〉 0.05 ). The 1 × 10^-10 - 1 × 10^-6 mol/L GnRH- Ⅱ could dose-dependently reduce VEGF protein secreted by endometrial stromal cells ( P 〈 0.01 ) , and the inhibition to ectopic endometrial stromal cells was stronger than that to eutopic endometrial stromal cells ( P 〈 0.01 ). Conclusion Ectopic stromal cells cultured in vitro can secrete VEGF, and so can eutopic stromal cells. This may play an important role in the formation and development of endometriosis. GnRH-Ⅱ can reduce VEGF protein secreted by ectopic endometrial stromal cells cultured in vitro, and its inhibition is stronger than that of eutopic endometrial stromal cells.
出处 《中南大学学报(医学版)》 CAS CSCD 北大核心 2009年第9期926-932,共7页 Journal of Central South University :Medical Science
关键词 子宫内膜异位症 Ⅱ型促性腺激素释放激素(GnRH-Ⅱ) 子宫内膜间质细胞 血管内皮生长因子 endometriosis gonadotropin-releasing hormone-Ⅱ endometrial stromal cell vascular endothelial growth factor
  • 相关文献

参考文献25

  • 1Donnez J, Pirard C, Smets M. Surgical management of endometriosis [ J ] . Best Pract Res Clin Obstet Gynaecol, 2004, 18(2) :329-348.
  • 2Enomoto M, Endo D, Kawashima S, et al. Human type Ⅱ GnRH receptor mediates effects of GnRH on cell proliferation [J].Zool Sci,2004,21(7) :763-770.
  • 3Grundker C, Gunthert A R, Millar R P, et al. Expression of gonadotropin- releasing hormone Ⅱ ( GnRH- Ⅱ ) receptor in human endometrial and ovarian cancer cells and effects of GnRH-Ⅱ on tumor cell proliferation [ J ]. J Clin Endocrinol Metab,2002,87 ( 3 ) : 1427-1430.
  • 4Wang A F, Li J H, Maiti K, et al. Preferential ligand selectivity of the monkey type- Ⅱ gonadotropin-releasing hormone (GnRH) receptor for GnRH-2 and its analogs [ J ]. Mol Cell Endocrinol, 2003 , 209 ( 1 -2 ) : 33 -42.
  • 5Mclaren J. Vascular endothelial growth factor and endometriotic angiogenesis [ J]. Hum Report Update,2000,6 (1) : 45 -55.
  • 6Wu M Y, Ho H N. The role of cytokines in endometriosis [J]. Am J Reprod Immunol,2003,49(5) :285-296.
  • 7秦莉花,秦明春,李晟,王若光,刘小丽,李春梅,吴长虹.人子宫内膜细胞原代体外培养方法[J].湖南中医药大学学报,2007,27(3):8-10. 被引量:13
  • 8Ryan I P, Schriock E D, Taylor R N. Isolation, characterization, and comparison of human endometrial and endornetriosis cells in vitro [ J ] . J Clin Endocrinol Metab, 1994 , 78 ( 3 ) : 642 -649.
  • 9Osteen K G, Hill G A, Hargrove J T, et al. Development of a method to isolate and culture highly purified populations of stromal and epithelial cells from human endometrial biopsy specimens[J]. Fertil Steril,1989,52(6) :965-972.
  • 10陈建林,林秋华,方小玲,陶光实,黄凤英.孕酮对异位子宫内膜间质细胞MMP-2和MMP-9表达的影响[J].中南大学学报(医学版),2005,30(3):307-311. 被引量:5

二级参考文献20

  • 1徐红,况燕,欧奇志,张玮.血管内皮生长因子在子宫内膜异位症中的作用[J].广西医科大学学报,2005,22(3):339-341. 被引量:5
  • 2郭海秀,陈向伟.VEGF在子宫内膜异位症中的表达[J].山西医科大学学报,2006,37(1):46-48. 被引量:4
  • 3[3]Akoum A.Jolicoeur C,Boucher A.Estradiol amplifies interleukin-1-induced monocyte chemotactic protein-1 expression by ectopic Endometrial cells of women with endometriosis[J].J Clin Endocrinol Metab,2000,85:896-904.
  • 4乐杰.妇产科学[M].6版.北京:人民卫生出版社,2003:154—158.
  • 5Wenzl R, Heinzl H. Localization of matrix metalloproteinase-2 in uterine endometrium and ectopic implants[J]. Gynecol Obstet Invest, 1998, 45(4): 253-257.
  • 6Korine I, Eeckhout Y, Nisolle M, et al. Expression of interstitial collagenase (matrix matalloproteinase-1) is related to the activity of human endometriotic lesions[J]. Fertil Steril, 1997, 68(2): 246-251.
  • 7Chung HW, Wen Y, Chun SH, et al. Matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-3 mRNA expression in ectopic and eutopic endometrium in women with endometriosis: a rationale for endometriotic invasiveness[J]. Fertil Steril, 2001,75(1): 152-159.
  • 8Overton CE, Fernandez-Shaw S, Hicks B, et al. In vitro culture of endometrial stromal and gland cells as a model for endometriosis: the effect of peritoneal fluid on proliferation[J]. Fertil Steril, 1997,67(1):51-56.
  • 9Kleiner DE, Stetler-Stevenson WG. Quantitative zymography: detection of picogram quantities of gelatinases[J]. Anal Biochem, 1994,218(2):325-329.
  • 10Bruner KL, Matrisian LM, Rodgers WH, et al. Suppression of matrix metalloproteinases inhibits establishment of ectopic lesions by human endometrium in nude mice[J]. J Clin Invest, 1997, 99(12):2851-2857.

共引文献15

同被引文献21

  • 1李华军,冷金花,郎景和,王惠兰,刘珠凤,孙大为,朱兰,丁小曼.子宫内膜异位症保守性手术后复发的相关因素分析[J].中华妇产科杂志,2005,40(1):13-16. 被引量:146
  • 2邓姗,李华军,郎景和,冷金花,刘珠凤,孙大为,朱兰.体内外孕激素和GnRHa对子宫内膜异位症患者在位内膜血管内皮生长因子表达影响的差异[J].中国妇产科临床杂志,2007,8(2):117-121. 被引量:12
  • 3蔡云朗,沈扬,任慕兰,赵维英,姚青,何杰,李嘉,翟卫中.米非司酮对子宫肌瘤组织中雌、孕激素受体及表皮生长因子受体的影响[J].现代医学,2007,35(4):299-303. 被引量:24
  • 4谢幸,苟文丽.妇产科学[M].8版.北京:人民卫生出版社,2013:118-119.
  • 5陈静.子宫内膜异位症发生机制研究进展[J].中华妇产科杂志,2012,38(7):105~109.
  • 6Donnez J. Vascular endothelial growth factor(VEGF) in endometrio- sis[J]. Hum Reprod, 2011,13(6) :1686 ~ 1690.
  • 7Donnez J,Smoes P,Gillerot S,et al.Vascular endothelial growth factor(VEGF)in endometriosis[J].Hum Reprod,2011,13(6):1686-1690.
  • 8Meresman GF,Bilotas M,Buquet RA,et al.Gonadotropin-releasing hormone agonist induces apoptosis and reduces cell proliferation in eutopic endometrial cultures from women with endometriosis[J].Fertil Setril,2010,80(Suppl 2):702-707.
  • 9Meresman GF,Bilotas MA,Lombardi E,et al.Eeffect of Gn RH analogues on apoptosis and release of interleukin-1βand rascular endothelial growth factor in endometrial cell cultures from patients with endometriosis[J].Hum Reprod,2011,18(6):1767-1771.
  • 10Saleh A, Tulandi T. Recperation after laparoscopic treaument of ovarian endometrionms by excision and by fenestration[J]. Fextil Sterll, 1999, 72(2): 322-324.

引证文献3

二级引证文献39

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部