摘要
目的:检测体外培养的子宫内膜异位症(endometriosis,EMs)患者在位和异位内膜间质细胞分泌的血管内皮生长因子(vascular endothelial growth factor,VEGF)的浓度,并观察不同浓度的II型促性腺激素释放激素(GnRH-Ⅱ)对在位和异位内膜间质细胞分泌VEGF的影响。方法:分别给予原代培养的EMs患者在位与异位子宫内膜间质细胞不同浓度(1×10-10~1×10-6mol/L)的GnRH-Ⅱ处理,不加GnRH-Ⅱ组作为对照,采用酶联免疫吸附法(ELISA)测定培养液中VEGF浓度并进行比较。结果:体外培养的EMs患者异位子宫内膜间质细胞分泌VEGF,培养48 h后分泌量与在位子宫内膜间质细胞比较差异无统计学意义(P(0.05)。1×10-10~1×10-6mol/L的GnRH-Ⅱ可呈浓度依赖性地抑制体外培养的在位和异位子宫内膜间质细胞分泌VEGF(P<0.01),且对异位子宫内膜间质细胞的抑制作用强于在位(P<0.01)。结论:EMs患者的异位子宫内膜间质细胞分泌VEGF的能力与在位子宫内膜的相近,这对EMs的形成和发展可能起重要作用。GnRH-Ⅱ对EMs患者体外培养的异位子宫内膜间质细胞VEGF的分泌有明显的抑制作用,且作用明显强于对在位内膜间质细胞的。
Objective To inspect the effect of gonadotropin-releasing hormonel Ⅱ ( GnRH-Ⅱ ) on the secretion of VEGF by eutopic and eetopic endometrial stromal cells cultured in vitro. Methods Eutopic and ectopic stromal cells cultured in vitro were treated with different concentrations (1 × 10^-10 -1 × 10^-6 mol/L)of GnRH-Ⅱ ,and the control group was not treated by GnRH-Ⅱ. Enzymelinked immunosorbent assay ( ELISA ) was used to measure the VEGF protein in the medium of the above 2 groups. Results There was no difference between the VEGF protein expressed by eutopic and ectopic stromal cells in the medium after culturing in vitro for 48 hours ( P 〉 0.05 ). The 1 × 10^-10 - 1 × 10^-6 mol/L GnRH- Ⅱ could dose-dependently reduce VEGF protein secreted by endometrial stromal cells ( P 〈 0.01 ) , and the inhibition to ectopic endometrial stromal cells was stronger than that to eutopic endometrial stromal cells ( P 〈 0.01 ). Conclusion Ectopic stromal cells cultured in vitro can secrete VEGF, and so can eutopic stromal cells. This may play an important role in the formation and development of endometriosis. GnRH-Ⅱ can reduce VEGF protein secreted by ectopic endometrial stromal cells cultured in vitro, and its inhibition is stronger than that of eutopic endometrial stromal cells.
出处
《中南大学学报(医学版)》
CAS
CSCD
北大核心
2009年第9期926-932,共7页
Journal of Central South University :Medical Science