应用混料实验设计制备秸秆复合降解菌剂

Application of Mixture Design for Compound Microbial Inoculants in Straw Degradation

农业秸秆类废弃物含有大量木质纤维素,该类物质结构稳定,不易降解,为秸秆的合理利用带来诸多困难。利用混料实验设计对筛选出可以共同培养的五种木质纤维素降解菌的配比进行研究,寻求复合发酵降解剂各组分的最佳配比,并分析发酵产品得到适用于不同发酵目的的菌剂。通过对发酵产品中木质素和纤维素降解率及还原糖的含量的分析建立模型,分析预测纤维素降解率最高为35.75%,木质素降解率最高为27%,还原糖含量最高为3.39mg/g。通过优化得出发酵菌剂最优配比为枯草芽胞杆菌12.1%,克鲁斯假丝酵母10%,地衣芽胞杆菌27.2%,变色栓菌10.6%,黄孢原毛平革菌40%。对应三指标的实测值为:35.47%,26.41%和2.37mg/g。

Crops straw contains large quantities of lignocelluloses that have stable structure and hardly degraded. Apply mixture design to study different mixtures of five strains that can degrade lignocelluloses. Regression model on microorganism composition and degrading rate of lignocelluloses and content of reducing sugar was established. The predictable degrading rate of cellulose reached 35.75 %. The maxim predictable degrading rate of lignin was 27% and the most predictable production of reducing sugar was 3.39mg/g. Based on these response values satisfied all expectation were optimized, and the most excellent combinations of Bacillus Subtilis , Bacillus Licheniformis , Canadian krusei, Trametes versicolor, Phaerochaete chrysosporium were 12.1% , 10% , 27.2% , 10.6% , and 40% , respectively. Three indicators of the measured values were 35.47% , 26.41% and 2.37mg/g.