survivin和livin在子痫前期胎盘中的表达及意义

The expressions of survivin and livin in placenta of pre-eclampsia and its significance

目的:检测抑凋亡基因survivin和livin在子痫前期患者胎盘的表达,研究滋养细胞凋亡与子痫前期的关系。方法:用免疫组化SP法检测胎盘组织中survivin和livin的表达,其中子痫前期20例、子痫4例、正常晚期妊娠20例,用计算机图像分析系统定量分析。结果:(1)survivin主要位于绒毛小叶的合体滋养细胞及细胞滋养细胞,在子痫前期的表达显著低于正常晚期妊娠(P<0.05),随病情加重表达降低;(2)livin主要在合体滋养细胞的细胞核中表达,在子痫前期、子痫组的表达均低于正常晚期妊娠(P<0.05);(3)子痫前期、子痫中survivin和livin表达无相关性(P>0.05)。结论:子痫前期及子痫患者胎盘组织中survivin、livin的表达均明显低于正常对照组,但两者无相关性(r=0.142,P=0.298,P>0.05)。

Objective： To detect the expression of survivin and livin in placenta of women with normal pregnancy, pre-eclampsia and eclampsia. Method：The expression of survivin and livin were collected from placenta of 20 pre-eclampsia patients,4 eclampsia patients and 20 normal pregnancy women which were determined by immunohistochemistry. The image analysis technique was employed to analyze livin and survivin. Results： Survivin was mostly found in syncytiotrophoblast, cytotrophoblast. Survivin was significantly lower than that in normal placenta. Livin was found in cell nucleus and cytoplasm of syncytiotrophoblasts. Livin protein in placental tissues of pre-eclampsia and eclampsia groups was significantly lower than that in norreal pregnancy group. The protein expression of livin and survivin did not correlate with each other（P 〉 0.05）. Conclusion：The abnormal expression of livin and Survivin in placental tissues may play an important role in the pathogenesis of pre-eclampsia and eclampsia.