摘要
目的:观察肝型脂肪酸结合蛋白(L-FABP)在大鼠非酒精性脂肪肝(NAFLD)模型中的动态表达,探讨L-FABP在大鼠NAFLD所起的作用。方法:选取Wistar大鼠40只,随机分为对照组和模型组。其中对照组给予普通饲料,模型组给予高脂饲料。分别于4,8,12周处死。测血清中的丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、血清胆固醇(TC)、血清甘油三酯(TG)。并测定空腹血糖(FBG)、空腹胰岛素(FINS),计算ISI。HE染色观察肝脏脂肪组织病理变化。并应用半定量反转录聚合酶链反应(RT-PCR)方法测定大鼠的肝脏组织中L-FABPmRNA的表达。结果:4周、8周1、2周模型组大鼠肝脏中L-FABPmRNA表达增强(P<0.01),且随着造模时间的延长,L-FABP的表达逐渐增高,与对照组差异显著(P<0.01)。模型组血清ALT、AST、TC、TG的水平均较对照组显著增高(P<0.01),而ISI明显降低(P<0.01)。结论:持续12周的高脂饮食可以成功复制大鼠NAFLD模型,且随着造模时间的延长可引起L-FABP表达逐渐增强。
Objective: To observe the dynamic expression of L-FABP in rat nonalcoholic fatty liver disease (NAFLD) model and to discuss the role of L-FABP in the rat NAFLD. Methods: Fourty Wistar rats(200 g - 220 g) were randomly divided into control group and model group, control group fed with standard diet; model group fed with high-fat diet(85% of the standard diet, 13% of lard and 2% cholesterol). The model group rat was put to death in 4,8,12 weeks seperately. Alanine aminotransferase in serum(ALT), aspartate aminotransferase in serum(AST), total cholesterol (TC), triglyeride (TG) ; mensurate fasting blood glucose ( FBG), Fasting insulin (FINS) were measured, and insulin sensitivity index ([SI) was calculated. HE staining was used to observe pathological changes in liver tissue; semi- quantitative reverse transcription polymerase Chain reaction (RT-PCR) method was used to determine the expression of L- FABPmRNA changes, in rat liver tissue. Results: The expression of L-FABPmRNA was gradually increased in the rat liver of model group in 4, 8 and 12 weeks( P 〈 0.01 ), the expression of L-FABP was gradually increased compared with the control group significantly( P 〈 0.01 ). In model group, the leral of serum ALT, AST, TC, TG was significantly higher than those in the control group ( P 〈 0.01 ), while ISI decreased significantly ( P 〈 0. 01 ). Conclusion: Continuous 12 weeks high-fat diet can successfully replicate NAFLD rat model, and with extending the diet feeding time the model caused the expression of L-FABP increases gradually.
出处
《中国实验方剂学杂志》
CAS
北大核心
2009年第9期64-66,共3页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
非酒精性脂肪肝
肝型脂肪酸结合蛋白
半定量反转录聚合酶链反应
non-alcoholic fatty liver
liver-fatty acid binding proteiniL-FABP)
reverse transcription polymerase chain reaction (RT-PCR)