肝型脂肪酸结合蛋白在大鼠非酒精性脂肪肝中的动态表达

The Dynamic Expression of Liver Fatty Acid Binding Protein in Rat Nonalcoholic Fatty Liver in Rat

目的:观察肝型脂肪酸结合蛋白(L-FABP)在大鼠非酒精性脂肪肝(NAFLD)模型中的动态表达,探讨L-FABP在大鼠NAFLD所起的作用。方法:选取Wistar大鼠40只,随机分为对照组和模型组。其中对照组给予普通饲料,模型组给予高脂饲料。分别于4,8,12周处死。测血清中的丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、血清胆固醇(TC)、血清甘油三酯(TG)。并测定空腹血糖(FBG)、空腹胰岛素(FINS),计算ISI。HE染色观察肝脏脂肪组织病理变化。并应用半定量反转录聚合酶链反应(RT-PCR)方法测定大鼠的肝脏组织中L-FABPmRNA的表达。结果:4周、8周1、2周模型组大鼠肝脏中L-FABPmRNA表达增强(P<0.01),且随着造模时间的延长,L-FABP的表达逐渐增高,与对照组差异显著(P<0.01)。模型组血清ALT、AST、TC、TG的水平均较对照组显著增高(P<0.01),而ISI明显降低(P<0.01)。结论:持续12周的高脂饮食可以成功复制大鼠NAFLD模型,且随着造模时间的延长可引起L-FABP表达逐渐增强。

Objective： To observe the dynamic expression of L-FABP in rat nonalcoholic fatty liver disease （NAFLD） model and to discuss the role of L-FABP in the rat NAFLD. Methods： Fourty Wistar rats（200 g - 220 g） were randomly divided into control group and model group, control group fed with standard diet; model group fed with high-fat diet（85% of the standard diet, 13% of lard and 2% cholesterol）. The model group rat was put to death in 4,8,12 weeks seperately. Alanine aminotransferase in serum（ALT）, aspartate aminotransferase in serum（AST）, total cholesterol （TC）, triglyeride （TG） ; mensurate fasting blood glucose （ FBG）, Fasting insulin （FINS） were measured, and insulin sensitivity index （[SI） was calculated. HE staining was used to observe pathological changes in liver tissue; semi- quantitative reverse transcription polymerase Chain reaction （RT-PCR） method was used to determine the expression of L- FABPmRNA changes, in rat liver tissue. Results： The expression of L-FABPmRNA was gradually increased in the rat liver of model group in 4, 8 and 12 weeks（ P 〈 0.01 ）, the expression of L-FABP was gradually increased compared with the control group significantly（ P 〈 0.01 ）. In model group, the leral of serum ALT, AST, TC, TG was significantly higher than those in the control group （ P 〈 0.01 ）, while ISI decreased significantly （ P 〈 0. 01 ）. Conclusion： Continuous 12 weeks high-fat diet can successfully replicate NAFLD rat model, and with extending the diet feeding time the model caused the expression of L-FABP increases gradually.