平肝潜阳方对肾血管性高血压大鼠CD62p和ICAM-1的影响

Effects of Principle of Restraining the Hyperactivity of the Liver Yang on Expressions of CD62p and ICAM-1 in the Renovascular Hypertensive Rats

目的:观察平肝潜阳方对肾血管性高血压大鼠脑血管内皮P-选择素(CD62p)和细胞间黏附分子-1(ICAM-1)表达的影响。方法:将50只SD大鼠随机分为正常对照组10只,肾血管性高血压大鼠40只,肾血管性高血压大鼠随机分为4组:模型组、平肝潜阳法组、阿司匹林组、平肝潜阳法加阿司匹林组,每组10只,从造模后12周连续给药4周,造模16周后每组取5只视交叉平面连续切片,每8张取1张切片作原位杂交检测脑血管内皮的ICAM-1、CD62p的mRNA水平,剩余5只取视交叉平面连续切片,每8张取1张切片作免疫组化。结果:平肝潜阳法组或阿司匹林组治疗后脑血管内皮的CD62pI、CAM-1原位杂交阳性信号和免疫组化的阳性信号较模型组明显减弱(P<0.05);平肝潜阳法加阿司匹林组CD62pI、CAM-1原位杂交阳性信号和免疫组化的阳性信号较阿司匹林组或平肝潜阳法组亦均有明显减弱(P<0.05)。结论:肾血管性高血压大鼠术后第16周末脑血管内皮CD62pI、CAM-1表达水平明显增高;平肝潜阳法有明显抑制脑血管内皮CD62pI、CAM-1表达的作用,平肝潜阳法与阿司匹林合用治疗效果更显著。

Objective：To observe the effects of the principle of restraining the hyperactivity of the liver yang on CD62p and ICAM-1 expressions in the cerebral vascular endothelium of renovascular hypertensive rats（RHR）. Methods： 50 rats were divided into control group （ n = 10） and renovascular hypertensive rats group （ n = 40）. 40 renovascular hypertensive rat were divided into four groups： model group, aspirin group, principle of restraining the hyperactivity of the liver yang group, principle of restraining the hyperactivity of the liver yang and aspirin group. The treatments Lasted 4 weeks after the establishment of the model. The rats were sacrificed and the tissues of the frontal lobe were slice into 5.0- μm-thick frozen sections from the bregma section underwent in situ hybridization of ICAM-1 and CD62p mRNA expressions, the other five rats were examined by histochemistry under electron microscope and light microscope. Results： After treating, the plasma levels of CD62p and ICAM-1 in the principle of restraining the hyperactivity of the liver yang or aspirin group were obviously lower than that in the model group, the Aspirin group and the principle restraining the hyperactivity of the liver yang group. ICAM-1 and CD62p mRNA expressions and immunohistochemistry expressions in the principle of restraining the hyperactivity of the liveryang and aspirin group were obviously lower than that in the aspirin group and the principle restraining the hyperactivity of the liver yang group. Conclusions： Expressions of CD62p and ICAM-1 in plasma are significantly high in RHR at 12 weeks after operation, principle of restraining the hyperactivity of the liver yang obviously repress the expressions of CD62p and ICAM-1 in cerebral vascular endothelium of renovascular hypertensive rats, and the effect of treatment with principle of restraining the hyperactivity of the liver yang and aspirin in the RHR is obviously improved.