细菌性肺炎痰热证模型的建立与评价

Establishment and Evaluation of Phlegm-heat Syndrome Models of Bacterial Pneumonia

目的建立、评价模拟临床证候的细菌性肺炎痰热证模型。方法采用气管插管细菌滴注法建立细菌性肺炎模型,并给予热环境风热刺激制备细菌性肺炎痰热证模型。将Wistar大鼠按随机数字表法分为正常对照组、肺炎组、热证组、模型组、方证对应组、方证非对应组,每组12只。观察大鼠一般状况、肺组织病理损伤,放射免疫法测定血肾上腺素(E)、去甲肾上腺素(NE)、皮质醇(Cor)及支气管肺泡灌洗液(BALF)中肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、细胞间黏附分子1(ICAM-1)的水平。结果与正常对照组比较,模型组、热证组、肺炎组、方证对应组、方证非对应组血中E、NE、Cor及BALF中TNF-α、IL-6、ICAM-1水平均升高(P<0.05或P<0.01),以模型组升高最明显(P<0.01)。与模型组比较,方证对应组、方证非对应组E、NE、Cor及BALF中TNF-α、IL-6、ICAM-1水平均下降,方证对应组下降更明显(P<0.05或P<0.01)。结论采用细菌滴注和风热刺激结合模型的方法建立细菌性肺炎痰热证模型是可行的。

Objective To set up and evaluate the anologous phlegm-heat syndrome models of bacterial pneumonia. Methods The bacterial pneumonia models were set up by bacteria instillation method through tracheal intubation, of them the phlegm heat syndrome models were made by giving wind heat stimulation in a hot environment. The rats were randomized into normal control group, pneumonia group, heat syndrome group, model group, recipe-manifestation corresponding group, and recipe-manifestation non-eorresponding group, with 12 rats in each. The general state of rats and pathological injury of lung tissues were observed. Radioimmunoassay was used to detect the blood epinephrine （E）, norepinephrine （NE）, cortisol （Cor）, and the contents of TNF-α, IL-6 and intercellular adhesion molecule 1 （ICAM-1） in the bronchoalveolar lavage fluid （BALF）. Results Compared with the normal control group, the blood E, NE, and Cor, and the contents of TNF-α, IL-6, and ICAM-1 in BALF of model group, heat syndrome group, pneumonia group, recipe-manifestation corresponding group, and recipe-manifestation non-corresponding group were all increased （P〈0.05 or P〈0.01）, with the most significant increase in the model group （P〈0.01L Compared with the model group, the blood E, NE, and Cor, and the contents of TNF-α, IL-6, and ICAM-1 in BALF of recipe-manifestation corresponding group and recipe-manifestation non-corresponding group were all decreased, with the most significant decrease in the recipe-manifestation corresponding group （P〈 0. 05 or P〈0. 01）. Conclusion To adopt the method of TCM etiology in combination with disease model to set up the heat syndrome model of bacterial pneumonia is practical.