HPLC法测定不同规格酸橙枳实中新橙皮苷和柚皮苷的含量

HPLC determination of neohesperidin and naringin in Fructus Aurantii Immaturus of different specifications

目的:建立枳实药材中新橙皮苷和柚皮苷的反相高效液相色谱测定方法,以考察不同规格酸橙枳实药材中新橙皮苷和柚皮苷的含量。方法:色谱柱Merck-Lichrospher RP-C18(4.6mm×250mm,5μm),流动相为乙腈-0.3%磷酸溶液(20∶80),流速1.0mL·min-1,检测波长283nm,柱温35℃。结果:新橙皮苷进样量在0.774～3.87μg范围内具有良好的线性关系(r=0.9997),平均回收率(n=6)为99.4%(RSD=1.3%);柚皮苷进样量在0.816～4.08μg范围内具有良好的线性关系(r=0.9997),平均回收率(n=6)为99.9%(RSD=1.6%)。不同规格酸橙枳实药材中新橙皮苷与柚皮苷含量分别为14.6%,7.02%(直径0.5～1.0cm);12.3%,9.78%(直径1.0～1.5cm);10.2%,10.2%(直径1.5～2.5cm)。结论:本方法准确,重现性好,简便易行,可有效地评价枳实药材的质量,为枳实药材的商品流通和开发研究提供可靠的依据。

Objective：To develop an RP - HPLC method for the determination of neohesperidin and naringin in Fructus Aurantii Immaturus of different specifications. Methods： The column was Merck- Lichrospher RP- Cls （4. 6 mm ×250 nm,5 μm） ;The mobile phase was a mixture of acetonitrile -0. 3% phosphoric acid（20： 80） ,at a flow rate of 1.0 mL ·min -1 ; The detection wavelength was 283 nm, and the column temperature was 35 ℃. Results：The calibration curves of neohesperidin, naringin were in good linearity over the ranges of 0. 774 -3.87 μg（r = 0. 9997 ） ,0. 816 - 4. 08 μg（ r = 0. 9997 ） , and the average recoveries （ n = 6 ） of neohesperidin and naringin were 99. 4% ,99.9% with RSD of 1.3%, 1.6% respectively. Neohesperidin and naringin contents in Fructus Aurantii Immaturus of different specifications were 14. 6% , 7.02% （ diameter 0. 5 - 1.0 cm） ; 12. 3%, 9. 78% （ diameter 1.0 - 1.5 cm） ; 10. 2%, 10. 2% （ diameter 1.5 - 2.5 cm ） respectively. Conclusion： This method is simple, sensi- tive,accurate,which will supply evidence for the quality control and research of Fructus Aurantii Immaturus.